Molecular detection of some virulence genes of E. coli isolated from broiler chickens and ducks at Ismailia governorate

Abstract EN

This study was conducted to investigate the existence of some virulence genes among Escherichia coli strains isolated from broiler chickens and ducks.

A total of 252 samples (liver, heart blood and lung) were collected from diseased and freshly dead broiler chickens (192 organ from 64 bird) and ducks (60 organ from 20 bird) from different poultry farms at Ismailia Governorate, Egypt during period from May 2018 to July 2019.

All samples were subjected to bacteriological examination for isolation and identification of E.

coli.

Eighteen E.

coli isolates were subjected for detection of some virulence genes (iss, iutA, papC, traT, eaeA and vat) by conventional polymerase chain reaction (cPCR).

Out of 252 samples, 203 (80.5%) were positive for E.

coli.

Virulence genes were detected among 18 selected E.

coli strains with variable percentage.

All 18 E.

coli isolates (100%) were iss, iutA and vat genes positive, 16 (88.8%) were traT gene positive, 5 (27.7%) were eaeA gene positive while, 2 (11%) were papC gene positive.