Antibiotic resistant patterns of bacteria recovered from infected diabetic foot as correlated to risk factors

Other Title(s)

أنماط مقاومة الجراثيم المعزولة من خمج قدم مرضى السكري و علاقتها ببعض عوامل الخطورة

Dissertant

Ali, Muhammad Shakir

Thesis advisor

al-Hadithi, Hadil Tawfiq

University

Isra University

Faculty

Faculty of Pharmacy

University Country

Jordan

Degree

Master

Degree Date

2020

English Abstract

Background: Incidence of diabetes is rising worldwide, and the prevalence of diabetes for all age-groups worldwide was estimated to be 2.8% in 2000 and 4.4% in 2030.

Contaminating bacteria among diabetic foot can couse foot ulceration and this can develop to gangarin then ampotation.

Aim of the study: Collecte Samples from diabetic foot infection (DFI) lesions at National Centre for diabetes endocrinology and genetics (NCDEG) diabetic foot clinic / University of Jordan, to isolate and identify contaminating bacteria, correlate their frequencies with risk factors, and study their antibiotics resistance patterns.

Patient and Methods: Seventy diabetic outpatients (Type II) aged above 30 years, with diabetic foot infection (DFI) attending NCDEG during the period from October 2019- Mid March 2020 were included in the study.

A structural questionnaire (Appendix A) was designed to collect data through interviewing patients in addition to medical records.

Swabs from DFI lesions were cultured onto a number of bacteriological media.

methicillin resistant Staphelococcus aureus (MRSA) and Methicillin-resistant staphylococcus aureus (MRCONS) were detected by growing isolates on Mannitol salt agar (MSA) and Mueller-Hinton agar supplemented with 6ug/ml Oxacillin.

An assortment of antibiotics against Gram negatev and Gram positive were examined to explore antibiotic resistance patterns.

Results:DFI are more prevalent in elderly patients aged > 50years (88.6%), male are more affected than females.

HbAIC was uncontrolled in 70.0% of patients and 47.1% of them have a duration of diabetes for more than 30 years.

Number of patients not committed to diet is 60% and 74.3% are non-smokers.

A significant correlation was found between HbAlC values with gender and diet (p<0.05).

A Similar incidence of DFI was reported by patients on either oral or insulin medication (17: 24.3% each).

36 patients (51.4%) were on both oral and insulin medication.

The duration of foot complication (28.6 %) of patients has lasted for more than 6 months.

Gram positive was the predominating bacteria isolated from DFI lesions.

S.aureus was the largest group (51.4%) followed by CONs (47.1%) and Enterococcus (12.9%).

MRSA and MRCONs were recovered from 40 % and 30% of study participants respectively.

The highest percentage recovery of MRSA and MRCONs were recorded among patients with duration of foot infection of 6 months - 1year (75% and 50% respectively).

Percentage frequencies of major Gram negative bacteria as following: Proteus mirabilis 18.6%, Pseudomonas aeruginosa 14.3%, and Acinetobacter spp.7.1%.

High frequencies of S.

aureus (55.6%), P.

aeruginosa (50%) and Kleb.

penumoniae (40%) were recovered among patients having surgical amputation.

Conculution: MDROs were prevalent amongst Gm+ve and Gm-ve isolates at alarming levels.

S.

aureus and Groub B streptococci (GBS) were resistant to seven and six antibiotics respectively.

Vancomycin, Linezolid, Teicoplanin and Tigecycline were the most effective against Gram positive organisms.

Gm-ve bacteria showed resistance to varied numbers (8-11)of antibiotics Only Prot.

mirabilis was resistant to Tigecycline (100%).

Main Topic

Pharmacology

No. of Pages

106

Table of Contents

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Introduction.

Chapter Two : Literature review.

Chapter Three : Research methodology.

Chapter Four : Results.

Chapter Five : Disscussin.

Chapter Six : Conclusion and recommendation.

References.

American Psychological Association (APA)

Ali, Muhammad Shakir. (2020). Antibiotic resistant patterns of bacteria recovered from infected diabetic foot as correlated to risk factors. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-985109

Modern Language Association (MLA)

Ali, Muhammad Shakir. Antibiotic resistant patterns of bacteria recovered from infected diabetic foot as correlated to risk factors. (Master's theses Theses and Dissertations Master). Isra University. (2020).
https://search.emarefa.net/detail/BIM-985109

American Medical Association (AMA)

Ali, Muhammad Shakir. (2020). Antibiotic resistant patterns of bacteria recovered from infected diabetic foot as correlated to risk factors. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-985109

Language

English

Data Type

Arab Theses

Record ID

BIM-985109