Fungal transformational studies on β-Caryophyllene and biological evaluation of the resulting metabolites
Other Title(s)
الدراسات التحويلية الفطرية على β-Caryophyllene و التقييم البيولوجي للأيضات الناتجة
Dissertant
al-Dulaymi, Qutaybah Ghazi Ibrahim
Thesis advisor
al-Najdawi, Manal
Muhammad, Yasin Muhammad
University
Isra University
Faculty
Faculty of Pharmacy
University Country
Jordan
Degree
Master
Degree Date
2020
English Abstract
Microbial transformations are organic reactions, catalyzed by microorganisms such as fungi.
They are often the key steps in the synthesis of many drug molecules, and natural products and their derivatives.
This technique has also been widely employed for the synthesis of new analogues of terpenoids.
During this work, a comparative microbial transformational study of selected terpenoid (β-caryophyllene) was carried out by various fungi to produce a new metabolite with interesting biological activities.
The transformed product was purified by using chromatographic procedures, and its structure was elucidated with the help of the spectroscopic techniques.
The transformed product was also evaluated for their biological activities, such as antioxidant and β-carotene bleaching assays.
β-Caryophyllene ((1R,4E,9S)-4,11,11-trimethyl-8-methylidenebicyclo[7.2.0]undec- 4ene, C15H24) (1), a bicyclicsesquiterpeneisolatedfromtheessentialoilsofthestems and the flowers of Syzgium aromaticum (cloves), and the essential oils of Cannabis sativa and Salvia rosmarinus (rosemary), has been shown to have anti-cancer, antioxidant, anti-inflammatoryand anti-bacterial activities.
Microbial transformation of β-caryophyllene (44), by suspended cell-cultures of the plant pathogenic fungus Aspergillus niger, resulted in the production of one metabolite.
The metabolite was identified as (3a,11a,E)-9-methyl-4- methylene3a,4,7,10,11,11a-hexahydro-2H-furo[3,2-c]oxecine2,6(3H)-dione (45), by differentspectroscopicmethods.Compound2wasfoundtobenew.
The antioxidant activity of β-caryophyllene (44), metabolite 45 and the standard was also studied on the basis of the radical scavenging effect of the stable DPPH free radical.
Compound 45 showed appreciable antioxidant activity.
Main Subjects
Topics
No. of Pages
65
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
[Chapter One] : Introduction.
[Chapter Two] : Experimental.
[Chapter Three] : Results and discussion.
[Chapter Four] : Conclusion.
[Chapter Five] : Future work.
References.
American Psychological Association (APA)
al-Dulaymi, Qutaybah Ghazi Ibrahim. (2020). Fungal transformational studies on β-Caryophyllene and biological evaluation of the resulting metabolites. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-985113
Modern Language Association (MLA)
al-Dulaymi, Qutaybah Ghazi Ibrahim. Fungal transformational studies on β-Caryophyllene and biological evaluation of the resulting metabolites. (Master's theses Theses and Dissertations Master). Isra University. (2020).
https://search.emarefa.net/detail/BIM-985113
American Medical Association (AMA)
al-Dulaymi, Qutaybah Ghazi Ibrahim. (2020). Fungal transformational studies on β-Caryophyllene and biological evaluation of the resulting metabolites. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-985113
Language
English
Data Type
Arab Theses
Record ID
BIM-985113
