Investigation of microbial contamination of in-use contact lens solutions correlated to habits and practices

Dissertant

al-Khani, Zahirah Murhaf Mustafa

Thesis advisor

al-Hadithi, Hadil Tawfiq

University

Isra University

Faculty

Faculty of Pharmacy

University Country

Jordan

Degree

Master

Degree Date

2020

English Abstract

Contact lenses (CLs) are medical or cosmetic device, comfortable and more convenient alternative to eyeglasses.

When microbes are inoculated onto CLs will result in eye infection because defense against microbial invasion in the anterior chamber of the eyes is weak due to the very poor blood supply.

The present study, investigates microbial contamination of CL cases, solutions and rims of solution bottles and assess habits and practices of thirty CL wearers toward lens care to understand factors associated with CL units contamination through a structured questionnaire.

Characteristics of grown colonies were determined through morphological and biochemical testing.

Five (16.6%) CL units were found not contaminated.

Eye redness after CLs wearing was almost statistically significant sign associated with CL units contamination (p=0.088).

Sixty four isolates were obtained; the most prevalent bacteria were Pseudomonas spp.(25%), Staphylococcus spp.

(21.9%), Serratia spp.

(14.1%), and Acinetobacter spp.

(6.2%).

Two types of CL solutions (A and B) exceeded required level of bacterial reduction (3 log) and reached up to 5 log reduction.

With regard to biofilms formation, after 4hr, tested solutions were able to reduce biofilm by more than 50% of all tested bacterial biofilms, regardless of the cleanness/dirtiness status.

Using water to wash hands and CL cases has been incriminated for increasing contamination.

Impurities in CL cases lead to reduced effectiveness of lens solutions.

Value-added awareness of CL wearer should be improved by regular visit to eye care professional

Main Topic

Pharmacology

No. of Pages

75

Table of Contents

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Introduction.

Chapter Two : Literature review.

Chapter Three : Methodology.

Chapter Four : Results.

Chapter Five : Discussion.

References.

American Psychological Association (APA)

al-Khani, Zahirah Murhaf Mustafa. (2020). Investigation of microbial contamination of in-use contact lens solutions correlated to habits and practices. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-985117

Modern Language Association (MLA)

al-Khani, Zahirah Murhaf Mustafa. Investigation of microbial contamination of in-use contact lens solutions correlated to habits and practices. (Master's theses Theses and Dissertations Master). Isra University. (2020).
https://search.emarefa.net/detail/BIM-985117

American Medical Association (AMA)

al-Khani, Zahirah Murhaf Mustafa. (2020). Investigation of microbial contamination of in-use contact lens solutions correlated to habits and practices. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-985117

Language

English

Data Type

Arab Theses

Record ID

BIM-985117