استحداث الكالس و استرجاع النبات من الأجنة الناضجة في الذرة البيضاء الحبية Sorghumbicolor L. Monech

Abstract EN

This Research was conducted in the laboratory of tissue culture affiliated to the National Commission of Biotechnology (NCBT) during the period between 2011 -2012 for induction of callus from mature embryos for three local Genotypes of grain Sorghum, regeneration of the plant from callus, rooting plantlets and acclimatization in order to get a plant capable to grow in greenhouse.

The best concentration for sterilizing the plant and entering it in use was 5% NaOCL for 20 minutes.

The addition of 2 mg/l of 2,4-D (2,4- dichlorophenoxyacetic acid) to MS medium caused an increase in the ratio of callus induction and embryogenic callus.

The regeneration and the number of (Thidiazuron), plantlets were raised after addition of 1.5 mg/l from TDZ (6-benzyl adenine) and 1mg/l from IAA(Indole-3-acetic 1.5mg/l from BAP (1-naphthylacetic acid) was the best in acid).

The addition of 1mg/l from NAA order to enhance both number and length of the roots.