MCP-1 −2518 AG Single Nucleotide Polymorphism in Slovak Patients with Systemic Sclerosis

Abstract EN

Recent study in a group of German patients with SSc has implicated the SNP in the MCP-1 gene (−2518 A to G) as a factor of susceptibility to SSc.

Reflecting the need for replication of genetic association studies, we investigated if this SNP is associated with SSc in another Caucasian population.

MCP-1 −2518 A/G genotypes were determined using PCR-SSP in 46 SSc patients and in 449 healthy subjects, all unrelated and of Slovak (Slavonic) origin.

The distribution of MCP-1 −2518 A/G genotypes complied with the Hardy-Weinberg equilibrium both in patient and healthy control groups.

There was no difference in MCP-1 −2518∗G allele frequency between SSc patients and healthy subjects (patients: 0.23; controls: 0.24; P>.05).

Furthermore, MCP-1 −2518 GG homozygotes were similarly represented among SSc patients and healthy subjects (P>.05).

The association of MCP-1 −2518 A/G SNP with SSc observed originally in German population was not replicated in the Slovak population.