The NF-κB Inhibitor Curcumin Blocks Sepsis-Induced Muscle Proteolysis
Joint Authors
Alamdari, Nima
Hasselgren, Per-Olof
O'Neal, Patrick
Menconi, Michael
Fareed, Moin U.
Reilly, Natasha
Poylin, Vitaliy Y.
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2007-01-09
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
We tested the hypothesis that treatment of rats with curcumin prevents sepsis-induced muscle protein degradation.
In addition, we determined the influence of curcumin on different proteolytic pathways that are activated in septic muscle (i.e., ubiquitin-proteasome-, calpain-, and cathepsin L-dependent proteolysis) and examined the role of NF-κB and p38/MAP kinase inactivation in curcumin-induced inhibition of muscle protein breakdown.
Rats were made septic by cecal ligation and puncture or were sham-operated.
Groups of rats were treated with three intraperitoneal doses (600 mg/kg) of curcumin or corresponding volumes of solvent.
Protein breakdown rates were measured as release of tyrosine from incubated extensor digitorum longus muscles.
Treatment with curcumin prevented sepsis-induced increase in muscle protein breakdown.
Surprisingly, the upregulated expression of the ubiquitin ligases atrogin-1 and MuRF1 was not influenced by curcumin.
When muscles from septic rats were treated with curcumin in vitro, proteasome-, calpain-, and cathepsin L-dependent protein breakdown rates were reduced, and nuclear NF-κB/p65 expression and activity as well as levels of phosphorylated (activated) p38 were decreased.
Results suggest that sepsis-induced muscle proteolysis can be blocked by curcumin and that this effect may, at least in part, be caused by inhibited NF-κB and p38 activities.
The results also suggest that there is not an absolute correlation between changes in muscle protein breakdown rates and changes in atrogin-1 and MuRF1 expression during treatment of muscle wasting.
American Psychological Association (APA)
Poylin, Vitaliy Y.& Fareed, Moin U.& O'Neal, Patrick& Alamdari, Nima& Reilly, Natasha& Menconi, Michael…[et al.]. 2007. The NF-κB Inhibitor Curcumin Blocks Sepsis-Induced Muscle Proteolysis. Mediators of Inflammation،Vol. 2008, no. 2008, pp.1-14.
https://search.emarefa.net/detail/BIM-988146
Modern Language Association (MLA)
Poylin, Vitaliy Y.…[et al.]. The NF-κB Inhibitor Curcumin Blocks Sepsis-Induced Muscle Proteolysis. Mediators of Inflammation No. 2008 (2008), pp.1-14.
https://search.emarefa.net/detail/BIM-988146
American Medical Association (AMA)
Poylin, Vitaliy Y.& Fareed, Moin U.& O'Neal, Patrick& Alamdari, Nima& Reilly, Natasha& Menconi, Michael…[et al.]. The NF-κB Inhibitor Curcumin Blocks Sepsis-Induced Muscle Proteolysis. Mediators of Inflammation. 2007. Vol. 2008, no. 2008, pp.1-14.
https://search.emarefa.net/detail/BIM-988146
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988146