Prolonged Classical NF-κB Activation Prevents Autophagy upon E. coli Stimulation In Vitro: A Potential Resolving Mechanism of Inflammation
Joint Authors
Meierhenrich, Rainer
Senftleben, Uwe
Stahl, Bettina
Georgieff, Michael
Schlottmann, Silke
Buback, Franziska
Walther, Paul
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-04-02
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Activation of NF-κB is known to prevent apoptosis but may also act as proapoptotic factor in order to eliminate inflammatory cells.
Here, we show that classical NF-κB activation in RAW 264.7 and bone marrow-derived macrophages upon short E.
coli coculture is necessary to promote cell death at late time points.
At 48 hours subsequent to short-term, E.
coli challenge increased survival of NF-κB-suppressed macrophages was associated with pattern of autophagy whereas macrophages with normal NF-κB signalling die.
Cell death of normal macrophages was indicated by preceding downregulation of autophagy associated genes atg5 and beclin1.
Restimulation of macrophages with LPS at 48 hours after E.
coli treatment results in augmented proinflammatory cytokine production in NF-κB-suppressed macrophages compared to control cells.
We thus demonstrate that classical NF-κB activation inhibits autophagy and promotes delayed programmed cell death.
This mechanism is likely to prevent the recovery of inflammatory cells and thus contributes to the resolution of inflammation.
American Psychological Association (APA)
Schlottmann, Silke& Buback, Franziska& Stahl, Bettina& Meierhenrich, Rainer& Walther, Paul& Georgieff, Michael…[et al.]. 2008. Prolonged Classical NF-κB Activation Prevents Autophagy upon E. coli Stimulation In Vitro: A Potential Resolving Mechanism of Inflammation. Mediators of Inflammation،Vol. 2008, no. 2008, pp.1-15.
https://search.emarefa.net/detail/BIM-988150
Modern Language Association (MLA)
Schlottmann, Silke…[et al.]. Prolonged Classical NF-κB Activation Prevents Autophagy upon E. coli Stimulation In Vitro: A Potential Resolving Mechanism of Inflammation. Mediators of Inflammation No. 2008 (2008), pp.1-15.
https://search.emarefa.net/detail/BIM-988150
American Medical Association (AMA)
Schlottmann, Silke& Buback, Franziska& Stahl, Bettina& Meierhenrich, Rainer& Walther, Paul& Georgieff, Michael…[et al.]. Prolonged Classical NF-κB Activation Prevents Autophagy upon E. coli Stimulation In Vitro: A Potential Resolving Mechanism of Inflammation. Mediators of Inflammation. 2008. Vol. 2008, no. 2008, pp.1-15.
https://search.emarefa.net/detail/BIM-988150
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988150