Effect of Oxysterol-Induced Apoptosis of Vascular Smooth Muscle Cells on Experimental Hypercholesterolemia
Joint Authors
Perales, Sonia
Iglesias, Jose
Palomino-Morales, Rogelio
Alejandre, M. José
Torres, Carolina
Linares, Ana
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-08-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Smooth muscle cells (SMCs) undergo changes related to proliferation and apoptosis in the physiological remodeling of vessels and in diseases such as atherosclerosis and restenosis.
Recent studies also have demonstrated the vascular cell proliferation and programmed cell death contribute to changes in vascular architecture in normal development and in disease.
The present study was designed to investigate the apoptotic pathways induced by 25-hydroxycholesterol in SMCs cultures, using an in vivo/in vitro cell model in which SMCs were isolated and culture from chicken exposed to an atherogenic cholesterol-rich diet (SMC-Ch) and/or an antiatherogenic fish oil-rich diet (SMC-Ch-FO).
Cells were exposed in vitro to 25-hydroxycholesterol to study levels of apoptosis and apoptotic proteins Bcl-2, Bcl-XL and Bax and the expression of bcl-2 and bcl-xL, genes.
The quantitative real-time reverse transcriptase-polymerase chain reaction and the Immunoblotting western blot analysis showed that 25-hydroxycholesterol produces apoptosis in SMCs, mediated by a high increase in Bax protein and Bax gene expression.
These changes were more marked in SMC-Ch than in SMC-Ch-FO, indicating that dietary cholesterol produces changes in SMCs that make them more susceptible to 25-hydroxycholesterol-mediated apoptosis.
Our results suggest that the replacement of a cholesterol-rich diet with a fish oil-rich diet produces some reversal of cholesterol-induced changes in the apoptotic pathways induced by 25-hydroxycholesterol in SMCs cultures, making SMCs more resistant to apoptosis.
American Psychological Association (APA)
Perales, Sonia& Alejandre, M. José& Palomino-Morales, Rogelio& Torres, Carolina& Iglesias, Jose& Linares, Ana. 2009. Effect of Oxysterol-Induced Apoptosis of Vascular Smooth Muscle Cells on Experimental Hypercholesterolemia. BioMed Research International،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988374
Modern Language Association (MLA)
Perales, Sonia…[et al.]. Effect of Oxysterol-Induced Apoptosis of Vascular Smooth Muscle Cells on Experimental Hypercholesterolemia. BioMed Research International No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-988374
American Medical Association (AMA)
Perales, Sonia& Alejandre, M. José& Palomino-Morales, Rogelio& Torres, Carolina& Iglesias, Jose& Linares, Ana. Effect of Oxysterol-Induced Apoptosis of Vascular Smooth Muscle Cells on Experimental Hypercholesterolemia. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988374
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988374