Identification of Four Entamoeba histolytica Organellar DNA Polymerases of the Family B and Cellular Localization of the Ehodp1 Gene and EhODP1 Protein
Joint Authors
Luna-Arias, Juan Pedro
Herrera-Aguirre, María Esther
Orozco, Esther
Labra-Barrios, María Luisa
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
We report the identification of a family of four active genes (Ehodp1, Ehodp2, Ehodp3, and Ehodp4) encoding putative DNA polymerases in Entamoeba histolytica, the protozoan parasite responsible of human amoebiasis.
The four Ehodp genes show similarity to DNA polymerases encoded in fungi and plant mitochondrial plasmids.
EhODP polypeptides conserve the 3′-5′ exonuclease II and 5′-3′ polymerization domains, and they have the I, II, and III conserved boxes that characterize them as DNA polymerases of family B.
Furthermore, we found in EhODP polymerases two novel A and B boxes, present also in DNA polymerases encoded in fungi mitochondrial plasmids.
By in situ PCR, Ehodp1 gene was located in nuclei and in DNA-containing cytoplasmic structures.
Additionally, using polyclonal antibodies against a recombinant rEhODP1-168 polypeptide, and confocal microscopy, EhODP1 was located in cytoplasmic DNA-containing structures.
American Psychological Association (APA)
Herrera-Aguirre, María Esther& Luna-Arias, Juan Pedro& Labra-Barrios, María Luisa& Orozco, Esther. 2010. Identification of Four Entamoeba histolytica Organellar DNA Polymerases of the Family B and Cellular Localization of the Ehodp1 Gene and EhODP1 Protein. BioMed Research International،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-989095
Modern Language Association (MLA)
Herrera-Aguirre, María Esther…[et al.]. Identification of Four Entamoeba histolytica Organellar DNA Polymerases of the Family B and Cellular Localization of the Ehodp1 Gene and EhODP1 Protein. BioMed Research International No. 2010 (Dec. 2010), pp.1-13.
https://search.emarefa.net/detail/BIM-989095
American Medical Association (AMA)
Herrera-Aguirre, María Esther& Luna-Arias, Juan Pedro& Labra-Barrios, María Luisa& Orozco, Esther. Identification of Four Entamoeba histolytica Organellar DNA Polymerases of the Family B and Cellular Localization of the Ehodp1 Gene and EhODP1 Protein. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-989095
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989095