Tocotrienol-Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts
Joint Authors
Mohd Yusof, Yasmin Anum
Makpol, Suzana
Kien Hui, Chua
Durani, Lina Wati
Wan Ngah, Wan Zurinah
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-23
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study determined the molecular mechanisms of tocotrienol-rich fraction (TRF) in preventing cellular senescence of human diploid fibroblasts (HDFs).
Primary culture of HDFs at various passages were incubated with 0.5 mg/mL TRF for 24 h.
Telomere shortening with decreased telomerase activity was observed in senescent HDFs while the levels of damaged DNA and number of cells in G0/G1 phase were increased and S phase cells were decreased.
Incubation with TRF reversed the morphology of senescent HDFs to resemble that of young cells with decreased activity of SA-β-gal, damaged DNA, and cells in G0/G1 phase while cells in the S phase were increased.
Elongated telomere length and restoration of telomerase activity were observed in TRF-treated senescent HDFs.
These findings confirmed the ability of tocotrienol-rich fraction in preventing HDFs cellular ageing by restoring telomere length and telomerase activity, reducing damaged DNA, and reversing cell cycle arrest associated with senescence.
American Psychological Association (APA)
Makpol, Suzana& Durani, Lina Wati& Kien Hui, Chua& Mohd Yusof, Yasmin Anum& Wan Ngah, Wan Zurinah. 2011. Tocotrienol-Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts. BioMed Research International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-990161
Modern Language Association (MLA)
Makpol, Suzana…[et al.]. Tocotrienol-Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts. BioMed Research International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-990161
American Medical Association (AMA)
Makpol, Suzana& Durani, Lina Wati& Kien Hui, Chua& Mohd Yusof, Yasmin Anum& Wan Ngah, Wan Zurinah. Tocotrienol-Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-990161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990161