The Over-expression of the β2 Catalytic Subunit of the Proteasome Decreases Homologous Recombination and Impairs DNA Double-Strand Break Repair in Human Cells
Joint Authors
Galli, Alvaro
Collavoli, Anita
Comelli, Laura
Cervelli, Tiziana
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
By a human cDNA library screening, we have previously identified two sequences coding two different catalytic subunits of the proteasome which increase homologous recombination (HR) when overexpressed in the yeast Saccharomyces cerevisiae.
Here, we investigated the effect of proteasome on spontaneous HR and DNA repair in human cells.
To determine if the proteasome has a role in the occurrence of spontaneous HR in human cells, we overexpressed the β2 subunit of the proteasome in HeLa cells and determined the effect on intrachromosomal HR.
Results showed that the overexpression of β2 subunit decreased HR in human cells without altering the cell proteasome activity and the Rad51p level.
Moreover, exposure to MG132 that inhibits the proteasome activity reduced HR in human cells.
We also found that the expression of the β2 subunit increases the sensitivity to the camptothecin that induces DNA double-strand break (DSB).
This suggests that the β2 subunit has an active role in HR and DSB repair but does not alter the intracellular level of the Rad51p.
American Psychological Association (APA)
Collavoli, Anita& Comelli, Laura& Cervelli, Tiziana& Galli, Alvaro. 2011. The Over-expression of the β2 Catalytic Subunit of the Proteasome Decreases Homologous Recombination and Impairs DNA Double-Strand Break Repair in Human Cells. BioMed Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990276
Modern Language Association (MLA)
Collavoli, Anita…[et al.]. The Over-expression of the β2 Catalytic Subunit of the Proteasome Decreases Homologous Recombination and Impairs DNA Double-Strand Break Repair in Human Cells. BioMed Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-990276
American Medical Association (AMA)
Collavoli, Anita& Comelli, Laura& Cervelli, Tiziana& Galli, Alvaro. The Over-expression of the β2 Catalytic Subunit of the Proteasome Decreases Homologous Recombination and Impairs DNA Double-Strand Break Repair in Human Cells. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990276
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990276