NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes

Joint Authors

Zhao, Yanhua
Choudhary, Sanjeev
Banerjee, Srijita
Tilton, Ronald G.
LeJeune, Wanda S.

Source

Journal of Diabetes Research

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-08-18

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Diseases
Medicine

Abstract EN

The impact of increased NF-κB-inducing kinase (NIK), a key component of the NF-κB activation pathways, on diabetes-induced renal inflammation remains unknown.

We overexpressed NIK wild type (NIKwt) or kinase-dead dominant negative mutants (NIKdn) in HK-2 cells and demonstrated that RelB and p52, but not RelA, abundance and DNA binding increased in nuclei of NIKwt but not NIKdn overexpressed cells, and this corresponded with increases in multiple proinflammatory cytokines.

Since TRAF3 negatively regulates NIK expression, we silenced TRAF3 by >50%; this increased nuclear levels of p52 and RelB, and transcript levels of proinflammatory cytokines and transcription factors.

In HK-2 cells and mouse primary proximal tubule epithelial cells treated with methylglyoxal-modified albumin, multiple proinflammatory cytokines and NIK were increased in association with increased nuclear RelB and p52.

These observations indicate that NIK regulates proinflammatory responses of renal proximal tubular epithelial cells via mechanisms involving TRAF3 and suggest a role for NF-κB noncanonical pathway activation in modulating diabetes-induced inflammation in renal tubular epithelium.

American Psychological Association (APA)

Zhao, Yanhua& Banerjee, Srijita& LeJeune, Wanda S.& Choudhary, Sanjeev& Tilton, Ronald G.. 2011. NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes. Journal of Diabetes Research،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-990620

Modern Language Association (MLA)

Zhao, Yanhua…[et al.]. NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes. Journal of Diabetes Research No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-990620

American Medical Association (AMA)

Zhao, Yanhua& Banerjee, Srijita& LeJeune, Wanda S.& Choudhary, Sanjeev& Tilton, Ronald G.. NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes. Journal of Diabetes Research. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-990620

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-990620