Ethanol Extract of Dianthus chinensis L. Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells In Vitro
Joint Authors
Nho, Kyoung Jin
Chun, Jin Mi
Kim, Ho Kyoung
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Dianthus chinensis L.
is used to treat various diseases including cancer; however, the molecular mechanism by which the ethanol extract of Dianthus chinensis L.
(EDCL) induces apoptosis is unknown.
In this study, the apoptotic effects of EDCL were investigated in human HepG2 hepatocellular carcinoma cells.
Treatment with EDCL significantly inhibited cell growth in a concentration- and time-dependent manner by inducing apoptosis.
This induction was associated with chromatin condensation, activation of caspases, and cleavage of poly (ADP-ribose) polymerase protein.
However, apoptosis induced by EDCL was attenuated by caspase inhibitor, indicating an important role for caspases in EDCL responses.
Furthermore, EDCL did not alter the expression of bax in HepG2 cells but did selectively downregulate the expression of bcl-2 and bcl-xl, resulting in an increase in the ratio of bax:bcl-2 and bax:bcl-xl.
These results support a mechanism whereby EDCL induces apoptosis through the mitochondrial pathway and caspase activation in HepG2 cells.
American Psychological Association (APA)
Nho, Kyoung Jin& Chun, Jin Mi& Kim, Ho Kyoung. 2012. Ethanol Extract of Dianthus chinensis L. Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992418
Modern Language Association (MLA)
Nho, Kyoung Jin…[et al.]. Ethanol Extract of Dianthus chinensis L. Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-992418
American Medical Association (AMA)
Nho, Kyoung Jin& Chun, Jin Mi& Kim, Ho Kyoung. Ethanol Extract of Dianthus chinensis L. Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells In Vitro. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992418
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992418