The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine
Joint Authors
Hu, Zhidong
Wan, Yanmin
Zhou, Haizhu
Wang, Jing
Ren, Xiaonan
Xu, Jianqing
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-01
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Previous studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine.
In this study, by constructing epitope-based DNA vaccines encoding a previously identified CD8+ T cell epitope, we investigated the influence of multiplying epitope copies on both the frequency and the MFI of specific IFN-γ secreting CD8+ T cells.
We found that frequencies of specific CD8+ T cell could be improved by multiplying epitope copies, while the MFI of IFN-γ secreted by epitope-specific CD8+ T cells decreased synchronously.
And further analysis showed that the decrease of MFI was not caused by the functional avidity variation of CD8+ T cell receptor.
American Psychological Association (APA)
Wan, Yanmin& Wang, Jing& Zhou, Haizhu& Hu, Zhidong& Ren, Xiaonan& Xu, Jianqing. 2012. The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-994441
Modern Language Association (MLA)
Wan, Yanmin…[et al.]. The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-994441
American Medical Association (AMA)
Wan, Yanmin& Wang, Jing& Zhou, Haizhu& Hu, Zhidong& Ren, Xiaonan& Xu, Jianqing. The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-994441
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-994441