Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants
Joint Authors
Wu, Ren-Chin
Wei, Fu-Chan
Wallace, Christopher Glenn
Huang, Wei-Chao
Lin, Jeng-Yee
Yang, Hsiang-Chen
Yen, Chia-Hung
Lin, Chun-Yen
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Mucosal or oral tolerance, an established method for inducing low-risk antigen-specific hyporesponsiveness, has not been investigated in vascularized composite allograft (VCA) research.
We studied its effects on recipient immune responses and VCA rejection.
Methods.
Lewis rats (n=12; TREATED) received seven daily intrajejunal treatments of 5×107 splenocytes from semiallogeneic Lewis-Brown-Norway rats (LBN) or vehicle (n=11; SHAM).
Recipients’ immune responses were assessed by mixed lymphocyte reaction (MLR) against donor antigen and controls.
Other Lewis (n=8; TREATED/VCA) received LBN hindlimb VCA and daily intrajejunal treatments of 5×107 LBN splenocytes, or LBN VCA without treatment (n=5; SHAM/VCA), until VCAs rejected.
Recipients’ immune responses were characterised and VCAs biopsied for histopathology.
Immunosuppressants were not used.
Results.
LBN-specific hyporesponsiveness was induced only in treated Lewis recipients.
Treatment significantly reduced MLR alloreactivity, significantly reduced VCA rejection on histopathology, and significantly delayed clinical VCA rejection (P<0.0005; TREATED/VCA mean 9.6 versus 6.0 days for SHAM/VCA).
Treatment significantly increased immunosuppressive IL-10/IL-4/TGF-β production and significantly decreased proinflammatory IFN-γ/TNF-α.
Conclusion.
Jejunal exposure to antigen conferred donor specific hyporesponsiveness that delayed VCA rejection.
This method may offer a low-risk adjunctive treatment option to help protect VCAs from rejection.
American Psychological Association (APA)
Wallace, Christopher Glenn& Yen, Chia-Hung& Yang, Hsiang-Chen& Lin, Chun-Yen& Wu, Ren-Chin& Huang, Wei-Chao…[et al.]. 2012. Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-996793
Modern Language Association (MLA)
Wallace, Christopher Glenn…[et al.]. Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-996793
American Medical Association (AMA)
Wallace, Christopher Glenn& Yen, Chia-Hung& Yang, Hsiang-Chen& Lin, Chun-Yen& Wu, Ren-Chin& Huang, Wei-Chao…[et al.]. Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-996793
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-996793