Changes in the Th1 : Th2 Cytokine Bias in Pregnancy and the Effects of the Anti-Inflammatory Cyclopentenone Prostaglandin 15-Deoxy-Δ12,14-Prostaglandin J2
Joint Authors
Sykes, Lynne
MacIntyre, David A.
Yap, Xiao J.
Ponnampalam, Sathana
Teoh, Tiong Ghee
Bennett, Phillip R.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Pregnancy is a complex immunological state in which a bias towards T helper 2 (Th2) protects the fetus.
Evidence suggests that proinflammatory cytokines increase the risk of poor neonatal outcome, independently of the direct effect of preterm labour.
The anti-inflammatory prostaglandin 15-deoxy-Δ12,14-Prostaglandin J2 (15dPGJ2) inhibits nuclear factor Kappa B (NF-κB) in amniocytes and myocytes in vitro and is a ligand for the chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) receptor.
Here we examine the Th1 : Th2 cytokine bias in pregnancy and whether 15dPGJ2 could be used to inhibit the production of the proinflammatory cytokines through inhibition of NF-κB while simultaneously promoting Th2 interleukin 4 (IL-4) synthesis via CRTH2 in T helper cells.
Peripheral blood mononuclear cells (PBMCs) from women at 28 weeks, term pre-labour, term labour as well as non-pregnant female controls were cultured with 15dPGJ2 or vehicle control and stimulated with phorbol myristyl acetate (PMA)/ionomycin.
The percentage of CD4+ cells producing interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in response to PMA/ionomycin was significantly reduced in pregnancy.
15dPGJ2 reduced IFN-γ and TNF-αproduction in stimulated T helper cells, but did not alter IL-4 production in CRTH2+ve cells.
15dPGJ2 also reduced phospho-p65 in stimulated PBMCs.
In summary, 15dPGJ2 suppresses the Th1 response of PBMCs during pregnancy and active labour whilst maintaining the Th2 response suggesting a therapeutic benefit in reducing neonatal morbidity in inflammation-induced PTL.
American Psychological Association (APA)
Sykes, Lynne& MacIntyre, David A.& Yap, Xiao J.& Ponnampalam, Sathana& Teoh, Tiong Ghee& Bennett, Phillip R.. 2012. Changes in the Th1 : Th2 Cytokine Bias in Pregnancy and the Effects of the Anti-Inflammatory Cyclopentenone Prostaglandin 15-Deoxy-Δ12,14-Prostaglandin J2. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-998915
Modern Language Association (MLA)
Sykes, Lynne…[et al.]. Changes in the Th1 : Th2 Cytokine Bias in Pregnancy and the Effects of the Anti-Inflammatory Cyclopentenone Prostaglandin 15-Deoxy-Δ12,14-Prostaglandin J2. Mediators of Inflammation No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-998915
American Medical Association (AMA)
Sykes, Lynne& MacIntyre, David A.& Yap, Xiao J.& Ponnampalam, Sathana& Teoh, Tiong Ghee& Bennett, Phillip R.. Changes in the Th1 : Th2 Cytokine Bias in Pregnancy and the Effects of the Anti-Inflammatory Cyclopentenone Prostaglandin 15-Deoxy-Δ12,14-Prostaglandin J2. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-998915
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998915