N-Acetyl Cysteine Protects against Methamphetamine-Induced Dopaminergic Neurodegeneration via Modulation of Redox Status and Autophagy in Dopaminergic Cells
المؤلفون المشاركون
Jin, Huajun
Anantharam, Vellareddy
Kanthasamy, Arthi
Chandramani Shivalingappa, Prashanth
Kanthasamy, Anumantha G.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-09-27
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Methamphetamine- (MA-) induced neurotoxicity is associated with mitochondrial dysfunction and enhanced oxidative stress.
Our previous study demonstrated that MA induces autophagy in a dopaminergic neuronal cell model (N27 cells).
The cellular mechanisms underlying MA-induced autophagy and apoptosis remain poorly characterized.
In the present study we sought to investigate the importance of GSH redox status in MA-induced neurotoxicity using a thiol antioxidant, N-acetylcysteine (NAC).
Morphological and biochemical analysis revealed that MA-induced autophagy in N27 dopaminergic cells was associated with pronounced depletion of GSH levels.
Moreover, pretreatment with NAC reduced MA-induced GSH depletion and autophagy, while depletion of GSH using L-buthionine sulfoximine (L-BSO) enhanced autophagy.
Furthermore, treatment with NAC significantly attenuated MA-induced apoptotic cell death as well as oxidative stress markers, namely, 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE).
Together, these results suggest that NAC exhibits significant protective effects against MA-induced dopaminergic cell death, presumably via modulation of the GSH level and autophagy.
Collectively, our data provide mechanistic insights into the role of cellular GSH redox status in MA-induced autophagy and apoptotic cell death, and additional studies are needed to determine the therapeutic effectiveness of cellular redox modifiers in attenuating dopaminergic neurodegeneration in vivo.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chandramani Shivalingappa, Prashanth& Jin, Huajun& Anantharam, Vellareddy& Kanthasamy, Anumantha G.& Kanthasamy, Arthi. 2012. N-Acetyl Cysteine Protects against Methamphetamine-Induced Dopaminergic Neurodegeneration via Modulation of Redox Status and Autophagy in Dopaminergic Cells. Parkinson’s Disease،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1002795
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chandramani Shivalingappa, Prashanth…[et al.]. N-Acetyl Cysteine Protects against Methamphetamine-Induced Dopaminergic Neurodegeneration via Modulation of Redox Status and Autophagy in Dopaminergic Cells. Parkinson’s Disease No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1002795
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chandramani Shivalingappa, Prashanth& Jin, Huajun& Anantharam, Vellareddy& Kanthasamy, Anumantha G.& Kanthasamy, Arthi. N-Acetyl Cysteine Protects against Methamphetamine-Induced Dopaminergic Neurodegeneration via Modulation of Redox Status and Autophagy in Dopaminergic Cells. Parkinson’s Disease. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1002795
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1002795
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر