Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation
المؤلفون المشاركون
Raynaud, C. M.
Maleki, M.
Lis, R.
Ahmed, B.
Al-Azwani, I.
Malek, J.
Safadi, F. F.
Tabrizi, Arash Rafii
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-04
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Mesenchymal stem cells (MSCs) are the most promising seed cells for cell therapy and can be isolated from various sources of human adult tissues such as bone marrow (BM-MSC) and adipose tissue.
However, cells from these tissues must be obtained through invasive procedures.
We, therefore, characterized MSCs isolated from fresh placenta (Pl-MSC) and fetal membrane (Mb-MSC) through morphological and fluorescent-activated cell sorting (FACS).
MSC frequency is higher in membrane than placenta (2.14% ± 0.65 versus 15.67% ± 0.29%).
Pl/Mb-MSCs in vitro expansion potential was significantly higher than BM-MSCs.
We demonstrated that one of the MSC-specific marker is sufficient for MSC isolation and that culture in specific media is the optimal way for selecting very homogenous MSC population.
These MSCs could be differentiated into mesodermal cells expressing cell markers and cytologic staining consistent with mature osteoblasts and adipocytes.
Transcriptomic analysis and cytokine arrays demonstrated broad similarity between placenta- and membrane-derived MSCs and only discrete differences with BM-MSCs with enrichment of networks involved in bone differentiation.
Pl/Mb-MSCs displayed higher osteogenic differentiation potential than BM-MSC when their response to osteoactivin was evaluated.
Fetal-tissue-derived mesenchymal cells may, therefore, be considered as a major source of MSCs to reach clinical scale banking in particular for bone regeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Raynaud, C. M.& Maleki, M.& Lis, R.& Ahmed, B.& Al-Azwani, I.& Malek, J.…[et al.]. 2012. Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation. Stem Cells International،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1003075
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Raynaud, C. M.…[et al.]. Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation. Stem Cells International No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1003075
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Raynaud, C. M.& Maleki, M.& Lis, R.& Ahmed, B.& Al-Azwani, I.& Malek, J.…[et al.]. Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1003075
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003075
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر