Labisia pumila Upregulates Peroxisome Proliferator-Activated Receptor Gamma Expression in Rat Adipose Tissues and 3T3-L1 Adipocytes
المؤلفون المشاركون
Mansor, Fazliana
Gu, Harvest F.
Östenson, Claes-Göran
Stener-Victorin, Elisabet
Wan Mohamud, Wan Nazaimoon
Mannerås-Holm, Louise
المصدر
Advances in Pharmacological and Pharmaceutical Sciences
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-07-07
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Peroxisome proliferator-activated receptor gamma (PPARgamma) is a ligand-activated transcription factor that regulates lipid and glucose metabolism.
We investigated the effects of Labisia pumila (LP) standardized water extract on PPARgamma transcriptional activity in adipocytes in vitro and in vivo.
We used a rat model of dihydrotestosterone- (DHT-) induced polycystic ovary syndrome (PCOS), a condition characterized by insulin resistance.
At 9 weeks of age, the PCOS rats were randomly subdivided into two groups: PCOS-LP (50 mg/kg/day of LP) and PCOS-control (1 mL of deionised water) for 4-5 weeks on the same schedule.
Real-time RT-PCR was performed to determine the PPARgamma mRNA levels.
LP upregulated PPARgamma mRNA level by 40% in the PCOS rats.
Western blot analysis further demonstrated the increased PPARgamma protein levels in parallel with upregulation in mRNA.
These observations were further proven by adipocytes culture.
Differentiated 3T3-L1 adipocytes were treated with final concentration of 100 μg/mL LP and compared to untreated control and 10 μM of rosiglitazone (in type of thiazolidinediones).
LP increased PPARgamma expressions at both mRNA and protein levels and enhanced the effect of glucose uptake in the insulin-resistant cells.
The data suggest that LP may ameliorate insulin resistance in adipocytes via the upregulation of PPARgamma pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mansor, Fazliana& Gu, Harvest F.& Östenson, Claes-Göran& Mannerås-Holm, Louise& Stener-Victorin, Elisabet& Wan Mohamud, Wan Nazaimoon. 2013. Labisia pumila Upregulates Peroxisome Proliferator-Activated Receptor Gamma Expression in Rat Adipose Tissues and 3T3-L1 Adipocytes. Advances in Pharmacological and Pharmaceutical Sciences،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003221
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mansor, Fazliana…[et al.]. Labisia pumila Upregulates Peroxisome Proliferator-Activated Receptor Gamma Expression in Rat Adipose Tissues and 3T3-L1 Adipocytes. Advances in Pharmacological and Pharmaceutical Sciences No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1003221
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mansor, Fazliana& Gu, Harvest F.& Östenson, Claes-Göran& Mannerås-Holm, Louise& Stener-Victorin, Elisabet& Wan Mohamud, Wan Nazaimoon. Labisia pumila Upregulates Peroxisome Proliferator-Activated Receptor Gamma Expression in Rat Adipose Tissues and 3T3-L1 Adipocytes. Advances in Pharmacological and Pharmaceutical Sciences. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003221
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003221
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر