Thermal and Chemical Stability of Two Homologous POZBTB Domains of KCTD Proteins Characterized by a Different Oligomeric Organization
المؤلفون المشاركون
Pirone, Luciano
Esposito, Carla
Correale, Stefania
Graziano, Giuseppe
Di Gaetano, Sonia
Vitagliano, Luigi
Pedone, Emilia
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-06
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
POZ/BTB domains are widespread modules detected in a variety of different biological contexts.
Here, we report a biophysical characterization of the POZ/BTB of KCTD6, a protein that is involved in the turnover of the muscle small ankyrin-1 isoform 5 and, in combination with KCTD11, in the ubiquitination and degradation of HDAC1.
The analyses show that the domain is a tetramer made up by subunits with the expected α/β structure.
A detailed investigation of its stability, carried out in comparison with the homologous pentameric POZ/BTB domain isolated from KCTD5, highlights a number of interesting features, which are shared by the two domains despite their different organization.
Their thermal/chemical denaturation curves are characterized by a single and sharp inflection point, suggesting that the denaturation of the two domains is a cooperative two-state process.
Furthermore, both domains present a significant content of secondary structure in their denatured state and a reversible denaturation process.
We suggest that the ability of these domains to fold and unfold reversibly, a property that is somewhat unexpected for these oligomeric assemblies, may have important implications for their biological function.
Indeed, these properties likely favor the formation of heteromeric associations that may be essential for the intricate regulation of the processes in which these proteins are involved.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pirone, Luciano& Esposito, Carla& Correale, Stefania& Graziano, Giuseppe& Di Gaetano, Sonia& Vitagliano, Luigi…[et al.]. 2013. Thermal and Chemical Stability of Two Homologous POZBTB Domains of KCTD Proteins Characterized by a Different Oligomeric Organization. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003577
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pirone, Luciano…[et al.]. Thermal and Chemical Stability of Two Homologous POZBTB Domains of KCTD Proteins Characterized by a Different Oligomeric Organization. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1003577
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pirone, Luciano& Esposito, Carla& Correale, Stefania& Graziano, Giuseppe& Di Gaetano, Sonia& Vitagliano, Luigi…[et al.]. Thermal and Chemical Stability of Two Homologous POZBTB Domains of KCTD Proteins Characterized by a Different Oligomeric Organization. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003577
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003577
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر