Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

الملخص EN

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well.

Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease.

In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines.

The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.