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The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo
المؤلفون المشاركون
Schageman, Jeoffrey
Zeringer, Emily
Li, Mu
Barta, Tim
Lea, Kristi
Gu, Jian
Magdaleno, Susan
Setterquist, Robert
Vlassov, Alexander V.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-25
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Exosomes are small (30–150 nm) vesicles containing unique RNA and protein cargo, secreted by all cell types in culture.
They are also found in abundance in body fluids including blood, saliva, and urine.
At the moment, the mechanism of exosome formation, the makeup of the cargo, biological pathways, and resulting functions are incompletely understood.
One of their most intriguing roles is intercellular communication—exosomes function as the messengers, delivering various effector or signaling macromolecules between specific cells.
There is an exponentially growing need to dissect structure and the function of exosomes and utilize them for development of minimally invasive diagnostics and therapeutics.
Critical to further our understanding of exosomes is the development of reagents, tools, and protocols for their isolation, characterization, and analysis of their RNA and protein contents.
Here we describe a complete exosome workflow solution, starting from fast and efficient extraction of exosomes from cell culture media and serum to isolation of RNA followed by characterization of exosomal RNA content using qRT-PCR and next-generation sequencing techniques.
Effectiveness of this workflow is exemplified by analysis of the RNA content of exosomes derived from HeLa cell culture media and human serum, using Ion Torrent PGM as a sequencing platform.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Schageman, Jeoffrey& Zeringer, Emily& Li, Mu& Barta, Tim& Lea, Kristi& Gu, Jian…[et al.]. 2013. The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo. BioMed Research International،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-1003807
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Schageman, Jeoffrey…[et al.]. The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo. BioMed Research International No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-1003807
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Schageman, Jeoffrey& Zeringer, Emily& Li, Mu& Barta, Tim& Lea, Kristi& Gu, Jian…[et al.]. The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-1003807
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003807
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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