In Vitro Characterization of a Multifunctional Staphylokinase Variant with Reduced Reocclusion, Produced from Salt Inducible E. coli GJ1158
المؤلفون المشاركون
Pulicherla, K. K.
Kumar, Anmol
Gadupudi, G. S.
Kotra, Seetha Ram
Sambasiva Rao, K. R. S.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-12
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
The thrombolytic therapy with clinically approved drugs often ensues with recurrent thrombosis caused by thrombin-induced platelet aggregation from the clot debris.
In order to minimize these problems, a staphylokinase (SAK)-based bacterial friendly multifunctional recombinant protein SRH (staphylokinase (SAK) linked with tripeptide RGD and dodecapeptide Hirulog (SRH)) was constructed to have Hirulog as an antithrombin agent and RGD (Arg-Gly-Asp) as an antiplatelet agent in the present study.
This multifunctional fusion protein SRH was expressed in osmotically inducible E.
coli GJ1158 as soluble form and purified with a yield of 0.27 g/L and functionally characterized in vitro.
SRH retained the fibrinolytic activity and plasminogen activation rate comparable to the parental counterpart SAK.
The antithrombin activity of SRH was significantly higher than SAK.
The platelet rich clot lysis assay indicated that SRH had enhanced platelet binding activity and T50% and C50 of SRH were significantly lower than that of SAK.
Furthermore, SRH inhibited the ADP-induced platelet aggregation in dose-dependent manner while SAK had no significant effect on platelet aggregation.
Thus, the current study suggests that the SAK variant produced from osmotically inducible GJ1158 is more potent thrombolytic agent with antithrombin and antiplatelet aggregation activities for reduction of reocclusion in thrombolytic therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pulicherla, K. K.& Kumar, Anmol& Gadupudi, G. S.& Kotra, Seetha Ram& Sambasiva Rao, K. R. S.. 2013. In Vitro Characterization of a Multifunctional Staphylokinase Variant with Reduced Reocclusion, Produced from Salt Inducible E. coli GJ1158. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1003938
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pulicherla, K. K.…[et al.]. In Vitro Characterization of a Multifunctional Staphylokinase Variant with Reduced Reocclusion, Produced from Salt Inducible E. coli GJ1158. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1003938
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pulicherla, K. K.& Kumar, Anmol& Gadupudi, G. S.& Kotra, Seetha Ram& Sambasiva Rao, K. R. S.. In Vitro Characterization of a Multifunctional Staphylokinase Variant with Reduced Reocclusion, Produced from Salt Inducible E. coli GJ1158. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1003938
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003938
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر