Release of a Wound-Healing Agent from PLGA Microspheres in a Thermosensitive Gel
المؤلفون المشاركون
Machado, H. A.
Abercrombie, J. J.
You, T.
DeLuca, P. P.
Leung, K. P.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-10-03
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The purpose of this research was to develop a topical microsphere delivery system in a thermosensitive 20% poloxamer 407 gel (Pluronic F127) to control release of KSL-W, a cationic antimicrobial decapeptide, for a period of 4–7 days for potential application in combat related injuries.
KSL-W loaded microsphere formulations were prepared by a solvent extraction-evaporation method (water-oil-water), with poly (D,L-lactic-co-glycolic acid) (PLGA) (50 : 50, low-weight, and hydrophilic end) as the polymeric system.
After optimization of the process, three formulations (A, B, and C) were prepared with different organic to water ratio of the primary emulsion while maintaining other components and manufacturing parameters constant.
Formulations were characterized for surface morphology, porous nature, drug loading, in vitro drug release, and antimicrobial activity.
Microspheres containing 20% peptide with porous surfaces and internal structure were prepared in satisfactory yields and in sizes varying from 25 to 50 μm.
Gels of 20% Pluronic F127, which were liquid at or below 24.6°C and formed transparent films at body temperature, were used as carriers for the microspheres.
Rheological studies showed a gelation temperature of 24.6°C for the 20% Pluronic F127 gel alone.
Gelation temperature and viscosity of formulations A, B, and C as a function of temperature were very close to those of the carrier.
A Franz diffusion cell system was used to study the release of peptide from the microspheres suspended in both, phosphate-buffered saline (PBS) and a 20% Pluronic F127 gel.
In vitro release of greater than 50% peptide was found in all formulations in both PBS and the gel, and in one formulation there was a release of 75% in both PBS and the gel.
Fractions collected from the release process were also tested for bactericidal activity against Staphylococcus epidermidis using the broth microdilution method and found to provide effective antimicrobial activity to warrant consideration and testing in animal wound models for treating combat-related injuries.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Machado, H. A.& Abercrombie, J. J.& You, T.& DeLuca, P. P.& Leung, K. P.. 2013. Release of a Wound-Healing Agent from PLGA Microspheres in a Thermosensitive Gel. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004169
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Machado, H. A.…[et al.]. Release of a Wound-Healing Agent from PLGA Microspheres in a Thermosensitive Gel. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1004169
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Machado, H. A.& Abercrombie, J. J.& You, T.& DeLuca, P. P.& Leung, K. P.. Release of a Wound-Healing Agent from PLGA Microspheres in a Thermosensitive Gel. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004169
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004169
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر