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Epithelial Cell Culture from Human Adenoids: A Functional Study Model for Ciliated and Secretory Cells
المؤلفون المشاركون
González, Claudia
Espinosa, Marisol
Sánchez, María Trinidad
Droguett, Karla
Ríos, Mariana
Fonseca, Ximena
Villalón, Manuel
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-02-05
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Mucociliary transport (MCT) is a defense mechanism of the airway.
To study the underlying mechanisms of MCT, we have both developed an experimental model of cultures, from human adenoid tissue of ciliated and secretory cells, and characterized the response to local chemical signals that control ciliary activity and the secretion of respiratory mucins in vitro.
Materials and Methods.
In ciliated cell cultures, ciliary beat frequency (CBF) and intracellular Ca2+ levels were measured in response to ATP, UTP, and adenosine.
In secretory cultures, mucin synthesis and secretion were identified by using immunodetection.
Mucin content was taken from conditioned medium and analyzed in the presence or absence of UTP.
Results.
Enriched ciliated cell monolayers and secretory cells were obtained.
Ciliated cells showed a basal CBF of 10.7 Hz that increased significantly after exposure to ATP, UTP, or adenosine.
Mature secretory cells showed active secretion of granules containing different glycoproteins, including MUC5AC.
Conclusion.
Culture of ciliated and secretory cells grown from adenoid epithelium is a reproducible and feasible experimental model, in which it is possible to observe ciliary and secretory activities, with a potential use as a model to understand mucociliary transport control mechanisms.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
González, Claudia& Espinosa, Marisol& Sánchez, María Trinidad& Droguett, Karla& Ríos, Mariana& Fonseca, Ximena…[et al.]. 2013. Epithelial Cell Culture from Human Adenoids: A Functional Study Model for Ciliated and Secretory Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1004400
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
González, Claudia…[et al.]. Epithelial Cell Culture from Human Adenoids: A Functional Study Model for Ciliated and Secretory Cells. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1004400
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
González, Claudia& Espinosa, Marisol& Sánchez, María Trinidad& Droguett, Karla& Ríos, Mariana& Fonseca, Ximena…[et al.]. Epithelial Cell Culture from Human Adenoids: A Functional Study Model for Ciliated and Secretory Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1004400
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004400
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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