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Development of an Ammonium Sulfate DNA Extraction Method for Obtaining Amplifiable DNA in a Small Number of Cells and Its Application to Clinical Specimens
المؤلفون المشاركون
Oh, Seo Young
Kim, Wook Youn
Hwang, Tae Sook
Han, Hye Seung
Lim, So Dug
Kim, Wan Seop
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-04-17
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
DNA extraction from microdissected cells has become essential for handling clinical specimens with advances in molecular pathology.
Conventional methods have limitations for extracting amplifiable DNA from specimens containing a small number of cells.
We developed an ammonium sulfate DNA extraction method (A) and compared it with two other methods (B and C).
DNA quality and quantity, β-globin amplification, and detectability of two cancer associated gene mutations were evaluated.
Method A showed the best DNA yield, particularly when the cell number was very low.
Amplification of the β-globin gene using DNA from the SNU 790 cell line and papillary thyroid carcinoma (PTC) cells extracted with Method A demonstrated the strongest band.
BRAFV600E mutation analysis using ethanol-fixed PTC cells from a patient demonstrated both a “T” peak increase and an adjacent “A” peak decrease when 25 and 50 cells were extracted, whereas mutant peaks were too low to be analyzed using the other two methods.
EGFR mutation analysis using formalin-fixed paraffin-embedded lung cancer tissues demonstrated a mutant peak with Method A, whereas the mutant peak was undetectable with Methods B or C.
Method A yielded the best DNA quantity and quality with outstanding efficiency, particularly when paucicellular specimens were used.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Oh, Seo Young& Kim, Wook Youn& Hwang, Tae Sook& Han, Hye Seung& Lim, So Dug& Kim, Wan Seop. 2013. Development of an Ammonium Sulfate DNA Extraction Method for Obtaining Amplifiable DNA in a Small Number of Cells and Its Application to Clinical Specimens. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1004574
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Oh, Seo Young…[et al.]. Development of an Ammonium Sulfate DNA Extraction Method for Obtaining Amplifiable DNA in a Small Number of Cells and Its Application to Clinical Specimens. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1004574
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Oh, Seo Young& Kim, Wook Youn& Hwang, Tae Sook& Han, Hye Seung& Lim, So Dug& Kim, Wan Seop. Development of an Ammonium Sulfate DNA Extraction Method for Obtaining Amplifiable DNA in a Small Number of Cells and Its Application to Clinical Specimens. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1004574
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004574
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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