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In Vitro Construction of Scaffold-Free Bilayered Tissue-Engineered Skin Containing Capillary Networks
المؤلفون المشاركون
Liu, Yuan
Luo, Hailang
Wang, Xinwen
Takemura, Akimichi
Fang, Yi Ru
Jin, Yan
Suwa, Fumihiko
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-27
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Many types of skin substitutes have been constructed using exogenous materials.
Angiogenesis is an important factor for tissue-engineered skin constructs.
In this study, we constructed a scaffold-free bilayered tissue-engineered skin containing a capillary network.
First, we cocultured dermal fibroblasts with dermal microvascular endothelial cells at a ratio of 2 : 1.
A fibrous sheet was formed by the interactions between the fibroblasts and the endothelial cells, and capillary-like structures were observed after 20 days of coculture.
Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue.
HE staining showed that tissue-engineered skin exhibited a stratified epidermis after 7 days.
Immunostaining showed that the epithelium promoted the formation of capillary-like structures.
Transmission electron microscopy (TEM) analysis showed that the capillary-like structures were typical microblood vessels.
ELISA demonstrated that vascularization was promoted by significant upregulation of vascularization associated growth factors due to interactions among the 3 types of cells in the bilayer, as compared to cocultures of fibroblast and endothelial cells and monocultures.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Yuan& Luo, Hailang& Wang, Xinwen& Takemura, Akimichi& Fang, Yi Ru& Jin, Yan…[et al.]. 2013. In Vitro Construction of Scaffold-Free Bilayered Tissue-Engineered Skin Containing Capillary Networks. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004586
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Yuan…[et al.]. In Vitro Construction of Scaffold-Free Bilayered Tissue-Engineered Skin Containing Capillary Networks. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1004586
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Yuan& Luo, Hailang& Wang, Xinwen& Takemura, Akimichi& Fang, Yi Ru& Jin, Yan…[et al.]. In Vitro Construction of Scaffold-Free Bilayered Tissue-Engineered Skin Containing Capillary Networks. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004586
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004586
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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