![](/images/graphics-bg.png)
Microscopic Evaluation, Molecular Identification, Antifungal Susceptibility, and Clinical Outcomes in Fusarium, Aspergillus and, Dematiaceous Keratitis
المؤلفون المشاركون
Gajjar, Devarshi U.
Pal, Anuradha K.
Ghodadra, Bharat K.
Vasavada, Abhay R.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-10-23
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Purpose.
Fusarium, Aspergillus, and Dematiaceous are the most common fungal species causing keratitis in tropical countries.
Herein we report a prospective study on fungal keratitis caused by these three fungal species.
Methodology.
A prospective investigation was undertaken to evaluate eyes with presumed fungal keratitis.
All the fungal isolates (n=73) obtained from keratitis infections were identified using morphological and microscopic characters.
Molecular identification using sequencing of the ITS region and antifungal susceptibility tests using microdilution method were done.
The final clinical outcome was evaluated in terms of the time taken for resolution of keratitis and the final visual outcome.
The results were analyzed after segregating the cases into three groups, namely, Fusarium, Aspergillus, and Dematiaceous keratitis.
Results.
Diagnosis of fungal keratitis was established in 73 (35.9%) cases out of 208 cases.
The spectra of fungi isolated were Fusarium spp.
(26.6%), Aspergillus spp.
(21.6%), and Dematiaceous fungi (11.6%).
The sequence of the ITS region could identify the Fusarium and Aspergillus species at the species complex level, and the Dematiaceous isolates were accurately identified.
Using antifungal agents such as fluconazole, natamycin, amphotericin B, and itraconazole, the minimum inhibitory concentrations (MICs) for Fusarium spp.
were >32 μg/mL, 4–8 μg/mL, 0.5–1 μg/mL, and >32 μg/mL, respectively.
Antifungal susceptibility data showed that Curvularia spp.
was highly resistant to all the antifungal agents.
Overall, natamycin and amphotericin B were found to be the most effective antifungal agents.
The comparative clinical outcomes in all cases showed that the healing response in terms of visual acuity of the Dematiaceous group was significantly good when compared with the Fusarium and Aspergillus groups (P<0.05).
The time required for healing in the Fusarium group was statistically significantly less when compared with the Aspergillus and Dematiaceous groups.
Conclusion.
This study demonstrates important differences in microscopic features of scraping material and antifungal susceptibility between the three groups.
Early and accurate identification coupled with the MIC data, and thereby appropriate treatment is crucial for complete recovery.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gajjar, Devarshi U.& Pal, Anuradha K.& Ghodadra, Bharat K.& Vasavada, Abhay R.. 2013. Microscopic Evaluation, Molecular Identification, Antifungal Susceptibility, and Clinical Outcomes in Fusarium, Aspergillus and, Dematiaceous Keratitis. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1004711
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gajjar, Devarshi U.…[et al.]. Microscopic Evaluation, Molecular Identification, Antifungal Susceptibility, and Clinical Outcomes in Fusarium, Aspergillus and, Dematiaceous Keratitis. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1004711
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gajjar, Devarshi U.& Pal, Anuradha K.& Ghodadra, Bharat K.& Vasavada, Abhay R.. Microscopic Evaluation, Molecular Identification, Antifungal Susceptibility, and Clinical Outcomes in Fusarium, Aspergillus and, Dematiaceous Keratitis. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1004711
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004711
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)