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Production, Purification, and Characterization of a Major Penicillium glabrum Xylanase Using Brewer's Spent Grain as Substrate
المؤلفون المشاركون
Knob, Adriana
Beitel, Susan Michelz
Fortkamp, Diana
Terrasan, César Rafael Fanchini
Almeida, Alex Fernando de
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-05-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
In recent decades, xylanases have been used in many processing industries.
This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate.
Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste.
Optimal production was obtained when P.
glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days.
The xylanase from P.
glabrum was purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography.
SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa.
The optimum activity was observed at 60 °C, in pH 3.0.
The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0.
The ion Mn2+ and the reducing agents β-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+ as well as the detergent SDS were strong inhibitors of the enzyme.
The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Knob, Adriana& Beitel, Susan Michelz& Fortkamp, Diana& Terrasan, César Rafael Fanchini& Almeida, Alex Fernando de. 2013. Production, Purification, and Characterization of a Major Penicillium glabrum Xylanase Using Brewer's Spent Grain as Substrate. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004973
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Knob, Adriana…[et al.]. Production, Purification, and Characterization of a Major Penicillium glabrum Xylanase Using Brewer's Spent Grain as Substrate. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1004973
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Knob, Adriana& Beitel, Susan Michelz& Fortkamp, Diana& Terrasan, César Rafael Fanchini& Almeida, Alex Fernando de. Production, Purification, and Characterization of a Major Penicillium glabrum Xylanase Using Brewer's Spent Grain as Substrate. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004973
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1004973
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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