Hypoxia and Human Genome Stability: Downregulation of BRCA2 Expression in Breast Cancer Cell Lines
المؤلفون المشاركون
Fanale, Daniele
Bazan, Viviana
Caruso, Stefano
Castiglia, Marta
Bronte, Giuseppe
Rolfo, Christian
Cicero, Giuseppe
Russo, Antonio
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Previously, it has been reported that hypoxia causes increased mutagenesis and alteration in DNA repair mechanisms.
In 2005, an interesting study showed that hypoxia-induced decreases in BRCA1 expression and the consequent suppression of homologous recombination may lead to genetic instability.
However, nothing is yet known about the involvement of BRCA2 in hypoxic conditions in breast cancer.
Initially, a cell proliferation assay allowed us to hypothesize that hypoxia could negatively regulate the breast cancer cell growth in short term in vitro studies.
Subsequently, we analyzed gene expression in breast cancer cell lines exposed to hypoxic condition by microarray analysis.
Interestingly, genes involved in DNA damage repair pathways such as mismatch repair, nucleotide excision repair, nonhomologous end-joining and homologous recombination repair were downregulated.
In particular, we focused on the BRCA2 downregulation which was confirmed at mRNA and protein level.
In addition, breast cancer cells were treated with dimethyloxalylglycine (DMOG), a cell-permeable inhibitor of both proline and asparaginyl hydroxylases able to induce HIF-1α stabilization in normoxia, providing results comparable to those previously described.
These findings may provide new insights into the mechanisms underlying genetic instability mediated by hypoxia and BRCA involvement in sporadic breast cancers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fanale, Daniele& Bazan, Viviana& Caruso, Stefano& Castiglia, Marta& Bronte, Giuseppe& Russo, Antonio…[et al.]. 2013. Hypoxia and Human Genome Stability: Downregulation of BRCA2 Expression in Breast Cancer Cell Lines. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005021
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Russo, Antonio…[et al.]. Hypoxia and Human Genome Stability: Downregulation of BRCA2 Expression in Breast Cancer Cell Lines. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1005021
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fanale, Daniele& Bazan, Viviana& Caruso, Stefano& Castiglia, Marta& Bronte, Giuseppe& Russo, Antonio…[et al.]. Hypoxia and Human Genome Stability: Downregulation of BRCA2 Expression in Breast Cancer Cell Lines. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005021
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1005021
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر