Human Resistin Inhibits Myogenic Differentiation and Induces Insulin Resistance in Myocytes
المؤلفون المشاركون
Sheng, Chun Hua
Du, Zhen Wu
Song, Yang
Wu, Xiao Dong
Zhang, Yu Cheng
Wu, Mei
Wang, Qian
Zhang, Gui Zhen
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-02-18
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
This study is aimed to investigate the effect of human resistin on myocyte differentiation and insulin resistance.
The human resistin eukaryotic expression vector was stable transfected into C2C12 myocyte cells and was transiently transfected into COS7 cells.
The effects of human resistin on cell proliferation, cell cycle, and myogenic differentiation of C2C12 cells were examined.
Glucose uptake assays was performed on C2C12 myotubes by using [3H] 2-deoxy-D-glucose.
The mRNA levels of insulin receptor (IR) and glucose transporter 4 (GLUT4) were evaluated by semiquantitative RT-PCR.
Results showed by the C2C12 cells transfected with human resistin gene compared with that without transfecting gene are as follows: (1) cell proliferation was significantly promoted, (2) after inducing differentiation, the myotube’s diameters and expression of desmin and myoglobin decreased, and (3) glucose uptake ratio was lowered and expression of IR and GLUT4 decreased.
However, there was no significant difference in the glucose uptake ratio between C2C12 myotubes treated with a human resistin conditioned medium of COS7 cells and treated with control medium.
These results suggest that maybe human resistin has not a direct role on insulin sensitivity of myocytes.
However, maybe it impaired the insulin sensitivity of myocytes through suppressing myogenesis and stimulating proliferation of myoblasts.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sheng, Chun Hua& Du, Zhen Wu& Song, Yang& Wu, Xiao Dong& Zhang, Yu Cheng& Wu, Mei…[et al.]. 2013. Human Resistin Inhibits Myogenic Differentiation and Induces Insulin Resistance in Myocytes. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005158
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sheng, Chun Hua…[et al.]. Human Resistin Inhibits Myogenic Differentiation and Induces Insulin Resistance in Myocytes. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1005158
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sheng, Chun Hua& Du, Zhen Wu& Song, Yang& Wu, Xiao Dong& Zhang, Yu Cheng& Wu, Mei…[et al.]. Human Resistin Inhibits Myogenic Differentiation and Induces Insulin Resistance in Myocytes. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005158
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1005158
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر