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Wound-Healing Potential of Cultured Epidermal Sheets Is Unaltered after Lyophilization: A Preclinical Study in Comparison to Cryopreserved CES
المؤلفون المشاركون
Jang, H.
Kim, Y. H.
Kim, M. K.
Lee, K. H.
Jeon, S.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-18
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Lyophilized Cultured Epidermal Sheets (L-CES) have been reported to be as effective as the cryopreserved CES (F-CES) in treating skin ulcers.
However, unlike F-CES, no preclinical study assessing wound-healing effects has been conducted for L-CES.
The present study was set out to investigate the microstructure, cytokine profile, and wound-healing effects of L-CES in comparison to those of F-CES.
Keratinocytes were cultured to prepare CES, followed by cryopreservation at −70°C and lyophilization.
Under microscopic observation, intact cells with apparent intracellular junctions were observed in L-CES.
The L-CES, like fresh CES, consisted of three to four well-maintained epidermal layers, as shown by the expression of keratins, involucrin, and p63.
There were no differences in the epidermal layer or protein expression between L-CES and F-CES, and both CES were comparable to fresh CES.
TGF-α, EGF, VEGF, IL-1α, and MMPs were detected in L-CES at levels similar to those in F-CES.
In a mouse study, wounds treated with L-CES or F-CES completely healed at least 4 days faster than untreated wounds.
CES-treated wounds completely healed by day 10, while the untreated wounds did not heal by day 14.
Masson’s trichrome staining showed that collagen deposition in the CES-treated wounds was highly increased in the dermis of the wound center compared to that in the control wounds.
Thus, this study demonstrates that L-CES is as clinically effective as F-CES for wound treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jang, H.& Kim, Y. H.& Kim, M. K.& Lee, K. H.& Jeon, S.. 2013. Wound-Healing Potential of Cultured Epidermal Sheets Is Unaltered after Lyophilization: A Preclinical Study in Comparison to Cryopreserved CES. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005381
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jang, H.…[et al.]. Wound-Healing Potential of Cultured Epidermal Sheets Is Unaltered after Lyophilization: A Preclinical Study in Comparison to Cryopreserved CES. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1005381
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jang, H.& Kim, Y. H.& Kim, M. K.& Lee, K. H.& Jeon, S.. Wound-Healing Potential of Cultured Epidermal Sheets Is Unaltered after Lyophilization: A Preclinical Study in Comparison to Cryopreserved CES. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005381
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1005381
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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