Detection of Mycobacterium tuberculosis by Using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick in Clinical Samples
المؤلفون المشاركون
Kaewphinit, Thongchai
Arunrut, Narong
Kiatpathomchai, Wansika
Santiwatanakul, Somchai
Jaratsing, Pornpun
Chansiri, Kosum
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-05
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Tuberculosis (TB) is a communicable disease caused by the bacterium Mycobacterium tuberculosis (MTB) and is a persistent problem in the developing countries.
Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature.
Here, a LAMP method was combined with a chromatographic lateral-flow dipstick (LFD) to detect IS6110 gene of M.
tuberculosis specifically and rapidly.
The reaction was optimized at 63°C for 60 min, and the amplified DNA hybridized to an FITC-labeled oligonucleotide probe for 5 min was detected at the LFD test line 5 min after application.
Excluding the step of DNA extraction, the test results could be generated approximately within 1 h.
In addition to the advantage of short assay time, this technique could avoid the contact of carcinogenic ethidium bromide due to the exclusion of the electrophoresis analysis step.
Furthermore, the data indicated that LAMP-LFD could detect M.
tuberculosis genomic DNA as little as 5 pg.
The technique showed a significant specificity since no cross-hybridization to M.
intracellulare (MIC), M.
fortuitum (MFT), M.
avium (MAV), M.
kansasii (MKS), and M.
gordonae (MGD) genomic DNAs was observed.
In the clinical unknown samples test, the sensitivity of LAMP-LFD was 98.92 % and the specificity was 100 % compared to those of the standard culture assay.
Based on its sensitivity, specificity, rapidity, low cost, and convenience, LAMP-LFD could be applicable for use in both laboratories and epidemiological surveys of MTB.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kaewphinit, Thongchai& Arunrut, Narong& Kiatpathomchai, Wansika& Santiwatanakul, Somchai& Jaratsing, Pornpun& Chansiri, Kosum. 2013. Detection of Mycobacterium tuberculosis by Using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick in Clinical Samples. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005433
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kaewphinit, Thongchai…[et al.]. Detection of Mycobacterium tuberculosis by Using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick in Clinical Samples. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1005433
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kaewphinit, Thongchai& Arunrut, Narong& Kiatpathomchai, Wansika& Santiwatanakul, Somchai& Jaratsing, Pornpun& Chansiri, Kosum. Detection of Mycobacterium tuberculosis by Using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick in Clinical Samples. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005433
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1005433
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر