Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum

المؤلفون المشاركون

Cao, Yan
Zhao, Bin
Han, Yanhui
Zhang, Juan
Li, Xuezhen
Qiu, Chunhui
Wu, Xiujuan
Hong, Yang
Ai, Dezhou
Lin, Jiaojiao
Fu, Zhiqiang

المصدر

BioMed Research International

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-12-30

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

Schistosomiasis, caused by infection with Schistosoma species, remains an important parasitic zoonosis.

Thioredoxin glutathione reductase of Schistosoma japonicum (SjTGR) plays an important role in the development of the parasite and for its survival.

Here we present a recombinant plasmid DNA vaccine, pVAX1/SjTGR, to estimate its protection against S.

japonicum in BALB/c mice.

The DNA vaccine administrated by particle bombardment induced higher protection than by intramuscular injection.

All animals vaccinated with pVAX1/SjTGR developed significant specific anti-SjTGR antibodies than control groups.

Moreover, animals immunized by gene gun exhibited a splenocyte proliferative response, with an increase in IFN-γ and IL-4.

The recombinant plasmid administrated by gene gun achieved a medium protective efficacy of 27.83–38.83% (P<0.01) of worm reduction and 40.38–44.51% (P<0.01) of liver egg count reduction.

It suggests that different modes of administering a DNA vaccine can influence the protective efficacy induced by the vaccine.

Interestingly, from the enzymatic activity results, we found that worms obtained from pVAX1/SjTGR-vaccinated animals expressed lower enzymatic activity than the control group and the antibodies weakened the enzymatic activity of SjTGR in vitro, too.

It implies that the high-level antibodies may contribute to the protective effects.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Cao, Yan& Zhao, Bin& Han, Yanhui& Zhang, Juan& Li, Xuezhen& Qiu, Chunhui…[et al.]. 2012. Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005499

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Cao, Yan…[et al.]. Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1005499

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Cao, Yan& Zhao, Bin& Han, Yanhui& Zhang, Juan& Li, Xuezhen& Qiu, Chunhui…[et al.]. Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005499

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1005499