In Vitro Wound Healing Potential and Identification of Bioactive Compounds from Moringa oleifera Lam

المؤلفون المشاركون

Muhammad, Abubakar Amali
Pauzi, Nur Aimi Syarina
Arulselvan, Palanisamy
Abas, Faridah
Fakurazi, Sharida

المصدر

BioMed Research International

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-12-31

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

Moringa oleifera Lam.

(M.

oleifera) from the monogeneric family Moringaceae is found in tropical and subtropical countries.

The present study was aimed at exploring the in vitro wound healing potential of M.

oleifera and identification of active compounds that may be responsible for its wound healing action.

The study included cell viability, proliferation, and wound scratch test assays.

Different solvent crude extracts were screened, and the most active crude extract was further subjected to differential bioguided fractionation.

Fractions were also screened and most active aqueous fraction was finally obtained for further investigation.

HPLC and LC-MS/MS analysis were used for identification and confirmation of bioactive compounds.

The results of our study demonstrated that aqueous fraction of M.

oleifera significantly enhanced proliferation and viability as well as migration of human dermal fibroblast (HDF) cells compared to the untreated control and other fractions.

The HPLC and LC-MS/MS studies revealed kaempferol and quercetin compounds in the crude methanolic extract and a major bioactive compound Vicenin-2 was identified in the bioactive aqueous fraction which was confirmed with standard Vicenin-2 using HPLC and UV spectroscopic methods.

These findings suggest that bioactive fraction of M.

oleifera containing Vicenin-2 compound may enhance faster wound healing in vitro.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Muhammad, Abubakar Amali& Pauzi, Nur Aimi Syarina& Arulselvan, Palanisamy& Abas, Faridah& Fakurazi, Sharida. 2013. In Vitro Wound Healing Potential and Identification of Bioactive Compounds from Moringa oleifera Lam. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005552

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Muhammad, Abubakar Amali…[et al.]. In Vitro Wound Healing Potential and Identification of Bioactive Compounds from Moringa oleifera Lam. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1005552

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Muhammad, Abubakar Amali& Pauzi, Nur Aimi Syarina& Arulselvan, Palanisamy& Abas, Faridah& Fakurazi, Sharida. In Vitro Wound Healing Potential and Identification of Bioactive Compounds from Moringa oleifera Lam. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005552

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1005552