Glycophenotype Evaluation in Cutaneous Tumors Using Lectins Labeled with Acridinium Ester
المؤلفون المشاركون
Lima, Luiza Rayanna Amorim
Bezerra, Matheus Filgueira
Almeida, Sinara Mônica Vitalino
Silva, Lúcia Patrícia Bezerra Gomes
de Carvalho Junior, Luiz Bezerra
Beltrão, Eduardo I. C.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-20
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Tumor cells show alterations in their glycosylation patterns when compared to normal cells.
Lectins can be used to evaluate these glycocode changes.
Chemiluminescence assay is an effective technique for quantitative analysis of proteins, nucleic acids, and carbohydrates due to its high sensitivity, specificity, and rapid testing.
Objective.
To use histochemiluminescence based on lectin conjugated to acridinium ester (AE) for the investigation of glycophenotype changes in cutaneous tumors.
Methods.
Concanavalin A (Con A), Peanut agglutinin (PNA), Ulex europaeus agglutinin-I (UEA-I), and Maackia amurensis agglutinin (MAA) were conjugated to acridinium ester.
Biopsies of cutaneous tumors and normal skin were incubated with the lectins-AE, and chemiluminescence was quantified and expressed as Relative Light Units (RLU).
Results.
Actinic keratosis (AK), keratoacanthoma (KA), squamous cell carcinoma (SCC), and basal cell carcinoma (BCC) showed lower expression of α-D-glucose/mannose and α-L-fucose residues compared to normal tissue.
Cutaneous tumors displayed higher expression of Gal-β(1-3)-GalNAc residues than normal tissue.
AK and SCC exhibited higher expression of Neu5Ac-α(2,3)Gal residues than normal epidermis.
KA and BCC showed equivalent RLU values compared to normal tissue.
Conclusions.
Lectin histochemiluminescence allowed quantitative assessment of the carbohydrate expression in cutaneous tissues, contributing to eliminate the subjectivity of conventional techniques used in the histopathological diagnosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lima, Luiza Rayanna Amorim& Bezerra, Matheus Filgueira& Almeida, Sinara Mônica Vitalino& Silva, Lúcia Patrícia Bezerra Gomes& Beltrão, Eduardo I. C.& de Carvalho Junior, Luiz Bezerra. 2013. Glycophenotype Evaluation in Cutaneous Tumors Using Lectins Labeled with Acridinium Ester. Disease Markers،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005873
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lima, Luiza Rayanna Amorim…[et al.]. Glycophenotype Evaluation in Cutaneous Tumors Using Lectins Labeled with Acridinium Ester. Disease Markers No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1005873
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lima, Luiza Rayanna Amorim& Bezerra, Matheus Filgueira& Almeida, Sinara Mônica Vitalino& Silva, Lúcia Patrícia Bezerra Gomes& Beltrão, Eduardo I. C.& de Carvalho Junior, Luiz Bezerra. Glycophenotype Evaluation in Cutaneous Tumors Using Lectins Labeled with Acridinium Ester. Disease Markers. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005873
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1005873
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر