Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry
المؤلفون المشاركون
Chen, Liyan
Luo, Zhenchao
Fu, Wenguang
Liao, Xinxin
Cui, Zhonglin
Zhou, Jie
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-23
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems.
Objective.
To detect urinary metabolomics after hepatic ischemia/reperfusion (I/R) injury induced by the Pringle maneuver using gas chromatography-mass spectrometry (GC-MS).
Methods.
Male Sprague-Dawley rats (N=80) were randomly divided into 4 groups (n=20/group): sham operation, day 1, day 3, and day 5.
Rats in the day 1, day 3, and day 5 groups underwent the Pringle maneuver.
Serum alanine transaminase (ALT) and total bilirubin (TBIL) were measured, and hematoxylin and eosin (HE) staining of the liver tissue was performed.
GC-MS was used to detect urinary metabolomics.
Results.
Compared with the sham group, the serum ALT and TBIL levels at day 1 were significantly elevated (P<0.01) and then decreased and reached close to normal levels at day 5.
GC-MS detected 7 metabolites which had similar changes as those of liver tissue revealed by histological examination.
Significant differences in lactic acid, pyruvic acid, alanine, serine, and glycerol-3-phosphate were found among the groups (P<0.001).
Principle component analysis showed that 7 metabolites distinguished the day 1 and day 3 groups from the sham group.
Conclusions.
Noninvasive urinary metabolomic analysis is a potential means for the early detection and diagnosis of hepatic I/R injury.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Liyan& Luo, Zhenchao& Fu, Wenguang& Liao, Xinxin& Cui, Zhonglin& Zhou, Jie. 2013. Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry. Disease Markers،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1005874
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Liyan…[et al.]. Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry. Disease Markers No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1005874
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Liyan& Luo, Zhenchao& Fu, Wenguang& Liao, Xinxin& Cui, Zhonglin& Zhou, Jie. Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry. Disease Markers. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1005874
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1005874
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر