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Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry
المؤلفون المشاركون
Chen, Liyan
Luo, Zhenchao
Fu, Wenguang
Liao, Xinxin
Cui, Zhonglin
Zhou, Jie
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-23
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems.
Objective.
To detect urinary metabolomics after hepatic ischemia/reperfusion (I/R) injury induced by the Pringle maneuver using gas chromatography-mass spectrometry (GC-MS).
Methods.
Male Sprague-Dawley rats (N=80) were randomly divided into 4 groups (n=20/group): sham operation, day 1, day 3, and day 5.
Rats in the day 1, day 3, and day 5 groups underwent the Pringle maneuver.
Serum alanine transaminase (ALT) and total bilirubin (TBIL) were measured, and hematoxylin and eosin (HE) staining of the liver tissue was performed.
GC-MS was used to detect urinary metabolomics.
Results.
Compared with the sham group, the serum ALT and TBIL levels at day 1 were significantly elevated (P<0.01) and then decreased and reached close to normal levels at day 5.
GC-MS detected 7 metabolites which had similar changes as those of liver tissue revealed by histological examination.
Significant differences in lactic acid, pyruvic acid, alanine, serine, and glycerol-3-phosphate were found among the groups (P<0.001).
Principle component analysis showed that 7 metabolites distinguished the day 1 and day 3 groups from the sham group.
Conclusions.
Noninvasive urinary metabolomic analysis is a potential means for the early detection and diagnosis of hepatic I/R injury.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Liyan& Luo, Zhenchao& Fu, Wenguang& Liao, Xinxin& Cui, Zhonglin& Zhou, Jie. 2013. Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry. Disease Markers،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1005874
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Liyan…[et al.]. Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry. Disease Markers No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1005874
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Liyan& Luo, Zhenchao& Fu, Wenguang& Liao, Xinxin& Cui, Zhonglin& Zhou, Jie. Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry. Disease Markers. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1005874
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1005874
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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