![](/images/graphics-bg.png)
Allergen-Specific IgG Antibodies Purified from Mite-Allergic Patients Sera Block the IgE Recognition of Dermatophagoides pteronyssinus Antigens: An In Vitro Study
المؤلفون المشاركون
Cunha-Júnior, Jair Pereira
de Aquino, Lais Martins
Silva, Deise Aparecida Oliveira
Miranda, Juliana Silva
Ynoue, Leandro Hideki
Siman, Isabella Lima
Pajuaba, Ana Claudia Arantes Marquez
Taketomi, Ernesto Akio
المصدر
Journal of Immunology Research
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-24
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
One of the purposes of specific immunotherapy (SIT) is to modulate humoral immune response against allergens with significant increases in allergen-specific IgG levels, commonly associated with blocking activity.
The present study investigated in vitro blocking activity of allergen-specific IgG antibodies on IgE reactivity to Dermatophagoides pteronyssinus (Dpt) in sera from atopic patients.
Dpt-specific IgG antibodies were purified by ammonium sulfate precipitation followed by protein-G affinity chromatography.
Purity was checked by SDS-PAGE and immunoreactivity by slot-blot and immunoblot assays.
The blocking activity was evaluated by inhibition ELISA.
The electrophoretic profile of the ammonium sulfate precipitated fraction showed strongly stained bands in ligand fraction after chromatography, compatible with molecular weight of human whole IgG molecule.
The purity degree was confirmed by detecting strong immunoreactivity to IgG, negligible to IgA, and no reactivity to IgE and IgM.
Dpt-specific IgG fraction was capable of significantly reducing levels of IgE anti-Dpt, resulting in 35%–51% inhibition of IgE reactivity to Dpt in atopic patients sera.
This study showed that allergen-specific IgG antibodies purified from mite-allergic patients sera block the IgE recognition of Dermatophagoides pteronyssinus antigens.
This approach reinforces that intermittent measurement of serum allergen-specific IgG antibodies will be an important objective laboratorial parameter that will help specialists to follow their patients under SIT.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Siman, Isabella Lima& de Aquino, Lais Martins& Ynoue, Leandro Hideki& Miranda, Juliana Silva& Pajuaba, Ana Claudia Arantes Marquez& Cunha-Júnior, Jair Pereira…[et al.]. 2013. Allergen-Specific IgG Antibodies Purified from Mite-Allergic Patients Sera Block the IgE Recognition of Dermatophagoides pteronyssinus Antigens: An In Vitro Study. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1006853
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Siman, Isabella Lima…[et al.]. Allergen-Specific IgG Antibodies Purified from Mite-Allergic Patients Sera Block the IgE Recognition of Dermatophagoides pteronyssinus Antigens: An In Vitro Study. Journal of Immunology Research No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1006853
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Siman, Isabella Lima& de Aquino, Lais Martins& Ynoue, Leandro Hideki& Miranda, Juliana Silva& Pajuaba, Ana Claudia Arantes Marquez& Cunha-Júnior, Jair Pereira…[et al.]. Allergen-Specific IgG Antibodies Purified from Mite-Allergic Patients Sera Block the IgE Recognition of Dermatophagoides pteronyssinus Antigens: An In Vitro Study. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1006853
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1006853
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)