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Immunohistochemical and Molecular Characterization of the Human Periosteum
المؤلفون المشاركون
Frey, Sönke Percy
Jansen, Hendrik
Doht, Stefanie
Filgueira, Luis
Zellweger, Rene
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-05-02
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Purpose.
The aim of the present study was to characterize the cell of the human periosteum using immunohistological and molecular methods.
Methods.
Phenotypic properties and the distribution of the cells within the different layers were investigated with immunohistochemical staining techniques and RT-PCR, focussing on markers for stromal stem cells, osteoblasts, osteoclasts and immune cells.
Results.
Immunohistochemical results revealed that all stained cells were located in the cambium layer and that most cells were positive for vimentin.
The majority of cells consisted of stromal stem cells and osteoblastic precursor cells.
The density increased towards the deeper layers of the cambium.
In addition, cells positive for markers of the osteoblast, chondrocyte, and osteoclast lineages were found.
Interestingly, there were MHC class II-expressing immune cells suggesting the presence of dendritic cells.
Using lineage-specific primer pairs RT-PCR confirmed the immunofluorescence microscopy results, supporting that human periosteum serves as a reservoir of stromal stem cells, as well as cells of the osteoblastic, and the chondroblastic lineage, osteoclasts, and dendritic cells.
Conclusion.
Our work elucidates the role of periosteum as a source of cells with a high regenerative capacity.
Undifferentiated stromal stem cells as well as osteoblastic precursor cells are dominating in the cambium layer.
A new outlook is given towards an immune response coming from the periosteum as MHC II positive immune cells were detected.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Frey, Sönke Percy& Jansen, Hendrik& Doht, Stefanie& Filgueira, Luis& Zellweger, Rene. 2013. Immunohistochemical and Molecular Characterization of the Human Periosteum. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1011977
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Frey, Sönke Percy…[et al.]. Immunohistochemical and Molecular Characterization of the Human Periosteum. The Scientific World Journal No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1011977
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Frey, Sönke Percy& Jansen, Hendrik& Doht, Stefanie& Filgueira, Luis& Zellweger, Rene. Immunohistochemical and Molecular Characterization of the Human Periosteum. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1011977
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1011977
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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