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Molecular and Virological Investigation of a Focal Chikungunya Outbreak in Northern India
المؤلفون المشاركون
Soni, Manisha
Singh, Anil Kumar
Sharma, Shashi
Agarwal, Ankita
Gopalan, Natarajan
Rao, P. V. Lakshmana
Parida, Manmohan
Dash, Paban K.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-24
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Chikungunya (CHIK) fever is one of the most important arboviral infections of medical significance.
The objective of the present study is to identify and characterize the etiology of a focal febrile arthritis outbreak from Gwalior, northern India, during October-November 2010.
A detailed virological (isolation) and molecular (end-point RT-PCR, quantitative RT-PCR, and nucleotide sequencing) investigation of this outbreak was carried out by collecting and studying 52 clinical samples and 15 mosquito pools from the affected region.
The investigation revealed the presence of CHIK viral RNA in 29% of clinical samples and 13% mosquito pool by RT-PCR.
The quantification of CHIK viral RNA in samples varied from 102.50 to 106.67 copies/mL, as demonstrated through quantitative RT-PCR.
In addition, six CHIK viruses were isolated from RT-PCR positive samples.
The nucleotide sequences of partial E1 gene of five representative CHIK viruses were deciphered, which revealed that all the viral strains from this outbreak belong to the recently emerging ECS African genotype.
Identification of Chikungunya virus ECSA African genotype as the etiology of the present outbreak confirms the continued circulation of the novel genotype, since 2006, in India.
The identification of CHIK virus in Aedes aegypti also confirmed it as the major vector in northern India.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Soni, Manisha& Singh, Anil Kumar& Sharma, Shashi& Agarwal, Ankita& Gopalan, Natarajan& Rao, P. V. Lakshmana…[et al.]. 2013. Molecular and Virological Investigation of a Focal Chikungunya Outbreak in Northern India. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1012051
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Soni, Manisha…[et al.]. Molecular and Virological Investigation of a Focal Chikungunya Outbreak in Northern India. The Scientific World Journal No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1012051
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Soni, Manisha& Singh, Anil Kumar& Sharma, Shashi& Agarwal, Ankita& Gopalan, Natarajan& Rao, P. V. Lakshmana…[et al.]. Molecular and Virological Investigation of a Focal Chikungunya Outbreak in Northern India. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1012051
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1012051
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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