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Purification and Characterization of Alkaline-Thermostable Protease Enzyme from Pitaya (Hylocereus polyrhizus) Waste: A Potential Low Cost of the Enzyme
المؤلفون المشاركون
Amid, Mehrnoush
ABD Manap, Mohd Yazid
Zohdi, Nor Khanani
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-09-18
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The thermoalkaline protease enzyme from pitaya (Hylocereus polyrhizus) waste was purified by a factor of 221.2 with 71.3% recovery using ammonium sulphate precipitation, gel filtration, and cation exchange chromatography.
Gel filtration chromatography together with sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) revealed that the enzyme is monomeric with a molecular weight of 26.7 kDa.
The apparent K m and V m a x of the protease were 2.8 mg/mL and 31.20 u/min, respectively.
The optimum pH and temperature were 8.0 and 70°C.
The enzyme was highly active and stable over a wide pH range (from pH 3.0 to pH 11.0 with the optimum activity at pH 8.0).
The protease has broad specificity toward azocasein, casein, hemoglobin, and gelatine.
Activity of the enzyme was inhibited by Fe2+ and Zn2+, while protease activity was increased in the presence of Ca2+ and Mg2+ and Cu2+ by factors of 125%, 110%, and 105%, respectively.
The alkaline protease showed extreme stability toward surfactants and oxidizing agent.
The purified protease exhibited extreme stability in the presence of organic solvents and inhibitors.
In addition, the enzyme was relativity stable toward organic solvents and chelating agents, such as ethylenediaminetetraacetic acid (EDTA).
The enzyme, derived from pitaya peel, possesses unique characteristics and could be used in various industrial and biotechnological applications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Amid, Mehrnoush& ABD Manap, Mohd Yazid& Zohdi, Nor Khanani. 2014. Purification and Characterization of Alkaline-Thermostable Protease Enzyme from Pitaya (Hylocereus polyrhizus) Waste: A Potential Low Cost of the Enzyme. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016186
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Amid, Mehrnoush…[et al.]. Purification and Characterization of Alkaline-Thermostable Protease Enzyme from Pitaya (Hylocereus polyrhizus) Waste: A Potential Low Cost of the Enzyme. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1016186
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Amid, Mehrnoush& ABD Manap, Mohd Yazid& Zohdi, Nor Khanani. Purification and Characterization of Alkaline-Thermostable Protease Enzyme from Pitaya (Hylocereus polyrhizus) Waste: A Potential Low Cost of the Enzyme. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016186
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1016186
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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