3D Dynamic Culture of Rabbit Articular Chondrocytes Encapsulated in Alginate Gel Beads Using Spinner Flasks for Cartilage Tissue Regeneration
المؤلفون المشاركون
Xu, Feiyue
Xu, Lei
Wang, Qi
Ye, Zhaoyang
Zhou, Yan
Tan, Wen-Song
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-11-24
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Cell-based therapy using chondrocytes for cartilage repair suffers from chondrocyte dedifferentiation.
In the present study, the effects of an integrated three-dimensional and dynamic culture on rabbit articular chondrocytes were investigated.
Cells (passages 1 and 4) were encapsulated in alginate gel beads and cultured in spinner flasks in chondrogenic and chondrocyte growth media.
Subcutaneous implantation of the cell-laden beads was performed to evaluate the ectopic chondrogenesis.
It was found that cells remained viable after 35 days in the three-dimensional dynamic culture.
Passage 1 cells demonstrated a proliferative growth in both media.
Passage 4 cells showed a gradual reduction in DNA content in growth medium, which was attenuated in chondrogenic medium.
Deposition of glycosaminoglycans (GAG) was found in all cultures.
While passage 1 cells generally produced higher amounts of GAG than passage 4 cells, GAG/DNA became similar on day 35 for both cells in growth media.
Interestingly, GAG/DNA in growth medium was greater than that in chondrogenic medium for both cells.
Based on GAG quantification and gene expression analysis, encapsulated passage 1 cells cultured in growth medium displayed the best ectopic chondrogenesis.
Taken together, the three-dimensional and dynamic culture for chondrocytes holds great potential in cartilage regeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Feiyue& Xu, Lei& Wang, Qi& Ye, Zhaoyang& Zhou, Yan& Tan, Wen-Song. 2014. 3D Dynamic Culture of Rabbit Articular Chondrocytes Encapsulated in Alginate Gel Beads Using Spinner Flasks for Cartilage Tissue Regeneration. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1016350
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Feiyue…[et al.]. 3D Dynamic Culture of Rabbit Articular Chondrocytes Encapsulated in Alginate Gel Beads Using Spinner Flasks for Cartilage Tissue Regeneration. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1016350
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Feiyue& Xu, Lei& Wang, Qi& Ye, Zhaoyang& Zhou, Yan& Tan, Wen-Song. 3D Dynamic Culture of Rabbit Articular Chondrocytes Encapsulated in Alginate Gel Beads Using Spinner Flasks for Cartilage Tissue Regeneration. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1016350
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1016350
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر