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Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress
المؤلفون المشاركون
Freitas, Isabel
Croce, Anna Cleta
Piccolini, Valeria Maria
Bertone, Vittorio
Bottiroli, Giovanni
Ferrigno, Andrea
Milanesi, Gloria
Tarantola, Eleonora
Boncompagni, Eleonora
Vairetti, Mariapia
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-09
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Oxidative stress in fatty livers is mainly generated by impaired mitochondrial β-oxidation, inducing tissue damages and disease progression.
Under suitable excitation, light liver endogenous fluorophores can give rise to autofluorescence (AF) emission, the properties of which depend on the organ morphofunctional state.
In this work, we characterized the AF properties of a rat liver model of lipid accumulation and oxidative stress, induced by a 1–9-week hypercaloric methionine-choline deficient (MCD) diet administration.
The AF analysis (excitation at 366 nm) was performed in vivo, via fiber optic probe, or ex vivo.
The contribution of endogenous fluorophores involved in redox reactions and in tissue organization was estimated through spectral curve fitting analysis, and AF results were validated by means of different histochemical and biochemical assays (lipids, collagen, vitamin A, ROS, peroxidised proteins, and lipid peroxidation -TBARS-, GSH, and ATP).
In comparison with the control, AF spectra changes found already at 1 week of MCD diet reflect alterations both in tissue composition and organization (proteins, lipopigments, and vitamin A) and in oxidoreductive pathway engagement (NAD(P)H, flavins), with a subsequent attempt to recover redox homeostasis.
These data confirm the AF analysis potential to provide a comprehensive diagnostic information on negative effects of oxidative metabolism alteration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Croce, Anna Cleta& Ferrigno, Andrea& Piccolini, Valeria Maria& Tarantola, Eleonora& Boncompagni, Eleonora& Bertone, Vittorio…[et al.]. 2014. Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1016565
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Croce, Anna Cleta…[et al.]. Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1016565
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Croce, Anna Cleta& Ferrigno, Andrea& Piccolini, Valeria Maria& Tarantola, Eleonora& Boncompagni, Eleonora& Bertone, Vittorio…[et al.]. Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1016565
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1016565
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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