Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from HypoxiaIschemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway
المؤلفون المشاركون
Yan, Xu
Tian, Jinwen
Wu, Hongjin
Liu, Yuna
Ren, Jianxun
Zheng, Sidao
Zhang, Chengying
Yang, Cui
Li, Yang
Wang, Shengqi
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-10
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Aim.
To investigate the effect of Ginsenoside Rb1 (GS-Rb1) on hypoxia/ischemia (H/I) injury in cardiomyocytes in vitro and the mitochondrial apoptotic pathway mediated mechanism.
Methods.
Neonatal rat cardiomyocytes (NRCMs) for the H/I groups were kept in DMEM without glucose and serum, and were placed into a hypoxic jar for 24 h.
GS-Rb1 at concentrations from 2.5 to 40 µM was given during hypoxic period for 24 h.
NRCMs injury was determined by MTT and lactate dehydrogenase (LDH) leakage assay.
Cell apoptosis, ROS accumulation, and mitochondrial membrane potential (MMP) were assessed by flow cytometry.
Cytosolic translocation of mitochondrial cytochrome c and Bcl-2 family proteins were determined by Western blot.
Caspase-3 and caspase-9 activities were determined by the assay kit.
Results.
GS-Rb1 significantly reduced cell death and LDH leakage induced by H/I.
It also reduced H/I induced NRCMs apoptosis induced by H/I, in accordance with a minimal reactive oxygen species (ROS) burst.
Moreover, GS-Rb1 markedly decreased the translocation of cytochrome c from the mitochondria to the cytosol, increased the Bcl-2/ Bax ratio, and preserved mitochondrial transmembrane potential (ΔΨm).
Its administration also inhibited activities of caspase-9 and caspase-3.
Conclusion.
Administration of GS-Rb1 during H/I in vitro is involved in cardioprotection by inhibiting apoptosis, which may be due to inhibition of the mitochondrial apoptotic pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yan, Xu& Tian, Jinwen& Wu, Hongjin& Liu, Yuna& Ren, Jianxun& Zheng, Sidao…[et al.]. 2014. Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from HypoxiaIschemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1018148
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yan, Xu…[et al.]. Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from HypoxiaIschemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1018148
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yan, Xu& Tian, Jinwen& Wu, Hongjin& Liu, Yuna& Ren, Jianxun& Zheng, Sidao…[et al.]. Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from HypoxiaIschemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1018148
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1018148
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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