Gene Expression Profile of Increased Heart Rate in Shensongyangxin-Treated Bradycardia Rabbits
المؤلفون المشاركون
Liu, Zhouying
Huang, Jian
Hu, Roumu
Huo, Youping
Gong, Jing
Zhang, Yinhui
Wei, Cong
Pu, Jielin
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-11-27
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Aims.
The present study tries to investigate the gene expression profile of bradycardia rabbits’ hearts after SSYX (SSYX, a traditional Chinese medicine) treatment.
Methods.
Eighteen adult rabbits were randomly assigned in three groups: sham, model, and SSYX treatment groups.
Heart rate was recorded in rabbits and total RNA was isolated from hearts.
Gene expression profiling was conducted and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed to confirm the gene expression results.
Patch clamp using human induced pluripotent stem cell-derived cardiomyocytes was applied to record the calcium current in the presence of SSYX.
Results.
The mean RR interval reduced after six weeks due to the injury of the sinoatrial node in the model group.
This effect was partially reversed by 4-week SSYX treatment.
cDNA microarray demonstrated that genes related with pacemaker current, calcium ion homeostasis, and signaling were altered by SSYX treatment.
Results from patch clamp demonstrated that SSYX reduced the calcium current which is consistent with gene expression results.
Conclusion.
The present study shows mRNA remodeling of bradycardia and demonstrates that SSYX is effective in treating bradycardia by reversing altered gene expression in bradycardia models.
Reduced calcium current by SSYX also confirmed the gene expression results.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Zhouying& Huang, Jian& Hu, Roumu& Huo, Youping& Gong, Jing& Zhang, Yinhui…[et al.]. 2014. Gene Expression Profile of Increased Heart Rate in Shensongyangxin-Treated Bradycardia Rabbits. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018946
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Zhouying…[et al.]. Gene Expression Profile of Increased Heart Rate in Shensongyangxin-Treated Bradycardia Rabbits. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1018946
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Zhouying& Huang, Jian& Hu, Roumu& Huo, Youping& Gong, Jing& Zhang, Yinhui…[et al.]. Gene Expression Profile of Increased Heart Rate in Shensongyangxin-Treated Bradycardia Rabbits. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018946
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1018946
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر