Taurine and caffeine-containing energy drinks : luminal presence of taurine reduces baclofen intestinal absorption in rats

الملخص EN

The absorption of baclofen (BAC), a centrally acting antipasti agent with γ-amino acid structure as well as the influence of turbine (TAU) and caffeine (CAFF) on baclofen permeability was investigated by in-situ single-pass rat intestinal perfusion.

Tourane is known to be transported by β-amino acid carrier, a transporter that is presumed to be shared by baclofen in its intestinal absorption.

The aim of this study was to characterize the intestinal transport properties of baclofen in three different intestinal segments and to demonstrate the influence of co-administration of turbine as a competitive inhibitor of active baclofen absorption.

Jejunum, ileum and colon of male White Westar rats (n = 4) were perfused simultaneously.

Baclofen concentration was 0.1 mM, a concentration, at which baclofen transport is not yet saturated.

Following 30 minutes perfusion with pure baclofen in tirade solution, 400mg / dl turbine (32 mmol / l), resp.

30 mg / dl caffeine (0.62 mmol / l) were added solely as well as in combination to the perfusion solution and the perfusion was continued in the same animal up to 90 minutes.

Tourane and caffeine concentrations corresponded to those usually used in energy drinks.

Additionally, baclofen permeability was directly studied using a typical energy drink (ED) Red Bull® instead of toyed.

The perforates were collected and baclofen concentrations were quantified by HPLC.

The intestinal permeability's (Peff) were determined on the basis of the differences between the inlet and outlet concentrations.

Results from in-situ intestinal perfusions in jejunum showed that Peff values are approximately reduced to 19.5 % following addition of turbine, 10.4 % by addition of turbine plus caffeine.

The energy drink reduced baclofen permeability to 13.9 %.

The effect of turbine and caffeine in ileum and colon was similar to those found in jejunum.