Mouse Mammary Tumor Virus Promoter-Containing Retroviral Promoter Conversion Vectors for Gene-Directed Enzyme Prodrug Therapy are Functional In Vitro and In Vivo
المؤلفون المشاركون
Ruttkowski, Bärbel
Schwab, Sonja
Peterbauer, Thomas
Günzburg, Walter H.
Hohenadl, Christine
Salmons, Brian
Klein, Reinhard
المصدر
العدد
المجلد 2008، العدد 2008 (31 ديسمبر/كانون الأول 2008)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2008-01-20
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Gene directed-enzyme prodrug therapy (GDEPT) is an approach for sensitization of tumor cells to an enzymatically activated, otherwise nontoxic, prodrug.
Cytochrome P450 2B1 (CYP2B1) metabolizes the prodrugs cyclophosphamide (CPA) and ifosfamide (IFA) to produce the cytotoxic substances phosphoramide mustard and isophosphoramide mustard as well as the byproduct acrolein.
We have constructed a retroviral promoter conversion (ProCon) vector for breast cancer GDEPT.
The vector allows expression of CYP2B1 from the mouse mammary tumor virus (MMTV) promoter known to be active in the mammary glands of transgenic animals.
It is anticipated to be used for the generation of encapsulated viral vector producing cells which, when placed inside or close to a tumor, will act as suppliers of the therapeutic CYP2B1 protein as well as of the therapeutic vector itself.
The generated vector was effectively packaged by virus producing cells and allowed the production of high levels of enzymatically active CYP2B1 in infected cells which sensitized them to killing upon treatment with both IFA and CPA.
Determination of the respective IC50 values demonstrated that the effective IFA dose was reduced by sixteen folds.
Infection efficiencies in vivo were determined using a reporter gene-bearing vector in a mammary cancer cell-derived xenograft tumor mouse model.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Klein, Reinhard& Ruttkowski, Bärbel& Schwab, Sonja& Peterbauer, Thomas& Salmons, Brian& Günzburg, Walter H.…[et al.]. 2008. Mouse Mammary Tumor Virus Promoter-Containing Retroviral Promoter Conversion Vectors for Gene-Directed Enzyme Prodrug Therapy are Functional In Vitro and In Vivo. BioMed Research International،Vol. 2008, no. 2008, pp.1-10.
https://search.emarefa.net/detail/BIM-1027037
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Klein, Reinhard…[et al.]. Mouse Mammary Tumor Virus Promoter-Containing Retroviral Promoter Conversion Vectors for Gene-Directed Enzyme Prodrug Therapy are Functional In Vitro and In Vivo. BioMed Research International No. 2008 (2008), pp.1-10.
https://search.emarefa.net/detail/BIM-1027037
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Klein, Reinhard& Ruttkowski, Bärbel& Schwab, Sonja& Peterbauer, Thomas& Salmons, Brian& Günzburg, Walter H.…[et al.]. Mouse Mammary Tumor Virus Promoter-Containing Retroviral Promoter Conversion Vectors for Gene-Directed Enzyme Prodrug Therapy are Functional In Vitro and In Vivo. BioMed Research International. 2008. Vol. 2008, no. 2008, pp.1-10.
https://search.emarefa.net/detail/BIM-1027037
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1027037
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر