Experimental Study of the Effects of Marrow Mesenchymal Stem Cells Transfected with Hypoxia-Inducible Factor-1α Gene
المؤلفون المشاركون
Li, Feng
Liu, Jian
Yang, Jinfu
Zhou, Xinmin
Zheng, Wei
Tang, Tao
Hu, Jianguo
Zhou, Wenwu
Yang, Yifeng
Tan, Zhiping
المصدر
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-07-01
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objective.
To construct the eukaryotic expression vector hypoxia-inducible factor 1α-pcDNA3.1 and to investigate its transfective efficiency into mesenchymal stem cells (MSCs) in vitro and the expression of HIF-1α gene in MSCs.
Methods.
mRNA of Wistar Rats' myocardial cells was extracted, and cDNA was synthesized with Reverse Transcription Kit, HIF-1α was amplified by polymerase chain reaction (PCR), and constructed into pcDNA3.1.
Transfected HIF-1α-pcDNA3.1 into MSCs by liposome mediated method.
The expression of HIF-1α in the cells was detected by Western Blot Analysis and ELISA.
Results.
Eukaryotic expression vector HIF-1α-pcDNA3.1 was constructed successfully.
Analyzed by flow cytometer, The MSCs' surfaces mark were CD44+, SH3(CD73)+, CD34−, CD45− and the CD44+ cells and SH3(CD73)+ cells were 94.7% and 97.3%, respectively, showing the high purity of the cultured MSCs.
After inducing, the cultured MSCs can differentiate into osteoblasts and adipocytes successfully.
In HIF-1α gene transfected MSCs, the expression of HIF-1α mRNA and HIF-1α protein were both increased obviously.
Conclusion.
HIF-1α was cloned successfully.
HIF-1α-pcDNA3.1 can be transfected into MSCs by liposome-mediated method effectively and which resulting stable expression ofHIF-1α in transfected MSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Jinfu& Tang, Tao& Li, Feng& Zhou, Wenwu& Liu, Jian& Tan, Zhiping…[et al.]. 2009. Experimental Study of the Effects of Marrow Mesenchymal Stem Cells Transfected with Hypoxia-Inducible Factor-1α Gene. BioMed Research International،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-1027080
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Jinfu…[et al.]. Experimental Study of the Effects of Marrow Mesenchymal Stem Cells Transfected with Hypoxia-Inducible Factor-1α Gene. BioMed Research International No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-1027080
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Jinfu& Tang, Tao& Li, Feng& Zhou, Wenwu& Liu, Jian& Tan, Zhiping…[et al.]. Experimental Study of the Effects of Marrow Mesenchymal Stem Cells Transfected with Hypoxia-Inducible Factor-1α Gene. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-1027080
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1027080
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر