In Vitro Study on Apoptosis Induced by Strontium-89 in HumanBreast Carcinoma Cell Line
المؤلفون المشاركون
Zhu, Min
Wang, Jing
Bao, Weiguang
Jiang, Han
Wang, Cheng
Chen, Baoguo
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-06-09
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Many radiopharmaceuticals used for medical diagnosis and therapyare beta emitters; however, the mechanism of the cell death causedby beta-irradiation is not well understood.
The objective of thisstudy was to investigate the apoptosis of human breast carcinomaMCF-7 cell lines induced by Strontium-89 (89Sr) and itsregulation and control mechanism.
High-metastatic Breast CarcinomaMCF-7 cells were cultured in vitro using 89Sr withdifferent radioactive concentration.
The inhibition rate of cellproliferation was measured by MTT color matching method.
The cellcycle retardation, apoptosis conditions, mitochondriontransmembrane potential difference and Fas expression were testedand analyzed.
The genes P53 and bcl-2 expressions was alsoanalyzed using immunity histochemical analysis.
After beinginduced by 89Sr with various of radioactiveconcentration, it was found that the inhibition of cellproliferation of MCF-7 cells was obviously, the retardation ofcell cycle occurred mainly in G2-M.
It was also found that theobvious apoptosis occurred after being induced by 89Sr,the highest apoptosis rate reached 46.28%.
The expressions ofFas acceptor and P53 gene increased, while bcl-2 gene expressiondecreasesd.
These findings demonstrate that in the ranges of acertain radioactive concentration, the inhibition rate of MCF-7cell proliferation and retardation of cell cycle had positivecorrelation with the concentration of 89Sr.
And themitochondrion transmembrane potential decrease would induce theapoptosis of MCF-7 cell notably, which were controlled by P53 andbcl-2 genes, involved with the Fas acceptor.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Cheng& Wang, Jing& Jiang, Han& Zhu, Min& Chen, Baoguo& Bao, Weiguang. 2011. In Vitro Study on Apoptosis Induced by Strontium-89 in HumanBreast Carcinoma Cell Line. BioMed Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-1027619
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Cheng…[et al.]. In Vitro Study on Apoptosis Induced by Strontium-89 in HumanBreast Carcinoma Cell Line. BioMed Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-1027619
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Cheng& Wang, Jing& Jiang, Han& Zhu, Min& Chen, Baoguo& Bao, Weiguang. In Vitro Study on Apoptosis Induced by Strontium-89 in HumanBreast Carcinoma Cell Line. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-1027619
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1027619
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر